Yellow fluorescent protein TurboYFP

- Super bright true-yellow fluorescence
- Emission wavelength is ideally positioned between those of green and red fluorescent proteins
- Fast maturation, high pH-stability and photostability
- Destabilized variant is available
- Recommended for gene expression analysis and cell and organelle labeling

Performance and use

Mammalian cells transiently transfected with TurboYFP expression vectors give bright fluorescense in 8-10 hrs after transfection. Being overexpressed in long-term culture of cells with high expression levels, TurboYFP shows slight tendency to aggregate. Therefore we recommend that you use parental PhiYFP and PhiYFP-m proteins for long-term expression and organelle labeling.

Despite there dimeric structure, TurboYFP and PhiYFPs perform well in some fusions. Please note, that PhiYFP can be used for generation of fusions to its N-terminus, whereas PhiYFP-m is optimized to generate fusions to its C-terminus. PhiYFP can not be used to generate C-terminal fusions. For protein labeling applications we recommend using specially optimized monomeric TagFPs.

TurboYFP can be used in multicolor labeling applications with blue, cyan, green, red, and far-red fluorescent dyes.

		TurboYFP expression in transiently transfected cells: (A) Phoenix cells; (B) Hela cells.