TagGFP


Green fluorescent protein TagGFP

- Fast protein maturation
- Bright green fluorescence
- Proven availability to generate stably transfected cell lines
- Recommended for protein labeling

TagGFP is an enhanced bright mutant of the Aequorea macrodactyla GFP-like protein [Xia et al., 2002]. TagGFP possesses bright green fluorescence with excitation/emission maxima at 482 and 505 nm, respectively. It is optimized for expression at 37oC. TagGFP is more pH-stable than EGFP.
Because of monomeric nature, TagGFP is mainly intended for protein localization studies and expression in long-term cell cultures. It can also be used for cell labeling and gene expression analysis, although TurboGFP is preferable for such applications because it matures faster and gives brighter fluorescent signal.

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TagGFP expression/source vectors

pTagGFP-CFP121Mammalian expression vector encoding humanazied TagGFP and allowing its expression and generation of fusions to the TagGFP C-terminus20 μg€ 400.00
pTagGFP-NFP122Mammalian expression vector encoding humanazied TagGFP and allowing its expression and generation of fusions to the TagGFP N-terminus20 μg€ 400.00
pTagGFP-actinFP124Mammalian expression vector encoding humanazied TagGFP fused with human cytoplasmic β-actin20 μg€ 400.00
pTagGFP-tubulinFP125Mammalian expression vector encoding humanazied TagGFP fused with human α-tubulin20 μg€ 400.00
pTagGFP-mitoFP127Mammalian expression vector encoding humanazied TagGFP targeted to mitochondria20 μg€ 400.00


Vector set

Fusion GreenFPF12Mammalian expression vectors encoding TagGFP for its expression and fusion generation: pTagGFP-C and pTagGFP-N20 μg each€ 650.00


Antibody against TagGFP

Anti-Tag(CGY)FP antibodyAB121Rabbit polyclonal antibody against TagCFP, TagGFP, TagYFP, PS-CFP2, and EGFP100 μg€ 150.00
AB122200 μg€ 200.00

References:

  • Xia NS, Luo WX, Zhang J, Xie XY, Yang HJ, Li SW, Chen M, Ng MH. Bioluminescence of Aequorea macrodactyla, a common jellyfish species in the East China Sea. Mar Biotechnol (NY). 2002; 4 (2):155-62. / pmid: 14961275
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