Anti-Tag(CGY)FP antibody

cat.# AB121, AB122

Rabbit polyclonal antibody against TagCFP, TagGFP, TagYFP, PS-CFP2, and EGFP

PRODUCT	CAT.#	SIZE	PRICE	DATA SHEET
Anti-Tag(CGY)FP antibody	AB121	100 μg	€ 150.00	AB121
	AB122	200 μg	€ 200.00	AB122
The prices do not include delivery. The prices vary in different countries. Please contact your local distributor for exact prices and delivery information.

Specificity
The antibody has been selected to recognize both denatured and native TagCFP, TagGFP, TagYFP, and PS-CFP2. The antibody also recognizes EGFP. The antibody shows little or no cross-reactivity with TagRFP, TurboGFP, TurboYFP, TurboRFP, TurboFP602, JRed, Dendra2, and KillerRed.

Western blot detection of fluorescent proteins using Anti-Tag(CGY)FP antibody. 1 — TagCFP; 2 — TagGFP; 3 — TagYFP; 4 — TagRFP; 5 — TurboFP602; 6 — TurboGFP; 7 — TurboYFP; 8 — TurboRFP; 9 — PS-CFP2; 10 — Dendra2; 11 — KillerRed; 12 — EGFP. Recombinant proteins were purified from transformed E. coli. 25 ng of each protein were separated by SDS PAGE (14% acrylamide). The samples were boiled before loading. Antibody was used at a 1/10000 dilution. Secondary antibody: Goat anti-Rabbit HRP-conjugated IgG.

Immunogen
Full-length recombinant denatured TagGFP comprising 6xHis tag.

Antibody preparation
Full-length recombinant TagGFP was purified from transformed E. coli using organic extraction and hydrophobic chromatography. Antibody was produced in rabbits immunized with the recombinant denatured TagGFP and purified by TagGFP affinity chromatography.

Formulation
Lyophilized from the buffer containing 0.01M Na₂PO₄, 0.1M NaCl, 0.25 mg/ml gelatin, and 1% trehalose; pH 7.4.

Reconstitution
Reconstitute with sterile water or 50% glycerol.

Storage
Lyophilized samples are stable for twelve months from date of receipt when stored at -20°C. The presence of silica gel drier is advisable.
Reconstituted with sterile water, antibody can be stored at +2 - +8°C for three months without detectable loss of activity.
Reconstituted with 50% glycerol, antibody can be stored at -20°C in a manual defrost freezer for six months without detectable loss of activity. Aliquot antibody upon reconstitution. Avoid repeated freeze / thaw cycles.

Use

• Western blot
• Immunoblotting
• Immunohistochemistry
• ELISA
• In cell Western
• Immunoprecipitation