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Cyan-to-green photoswitchable fluorescent protein PS-CFP2
- Irreversible photoconversion from a cyan to a green fluorescent form
- High contrast of photoconversion
- High pH stability allowing labeling of acidic organelles
- Recommended for tracking cell, organelle, and protein movement, and for determination of protein half-life
Protein description | PS-CFP2 is an improved mutant of the photoswitchable monomeric fluorescent protein PS-CFP [Chudakov et al., 2004]. PS-CFP2 exhibits faster maturation and a brighter fluorescence both before and after photoswitching than that of PS-CFP.
PS-CFP2 is capable of irreversible photoconversion from cyan to green fluorescent form in response to 405 nm light irradiation. It is recommended for real-time in vivo tracking movement of cells, organelles, and individual proteins [Chudakov et al., 2007]. It can also be applied for determination of protein half-life [Zhang et al., 2007]. In addition, PS-CFP2 can be used as a routine cyan fluorescent tag (excitation maximum at 400 nm and emission maximum at 468 nm) at moderate excitation intensities and as a donor in FRET applications [Souslova and Chudakov, 2006]. |
Related products| PRODUCT | CAT.# | DESCRIPTION | SIZE | PRICE | | The prices do not include delivery. The prices vary in different countries. Please contact your local distributor for exact prices and delivery information. |
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| PS-CFP2 expression/source vectors |
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| pPS-CFP2-C | FP801 | Mammalian expression vector encoding humanazied PS-CFP2 and allowing its expression and generation of fusions to the PS-CFP2 C-terminus | 20 μg | € 400.00 | | pPS-CFP2-N | FP802 | Mammalian expression vector encoding humanazied PS-CFP2 and allowing its expression and generation of fusions to the PS-CFP2 N-terminus | 20 μg | € 400.00 |
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| Antibody against PS-CFP2 |
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| Anti-Tag(CGY)FP antibody | AB121 | Rabbit polyclonal antibody against TagCFP, TagGFP, TagYFP, PS-CFP2, and EGFP | 100 μg | € 150.00 | | AB122 | 200 μg | € 200.00 |
References:
- Chudakov DM, Lukyanov S, Lukyanov KA. Tracking intracellular protein movements using photoswitchable fluorescent proteins PS-CFP2 and Dendra2. Nat Protoc. 2007; 2 (8):2024-32. / pmid: 17703215
- Chudakov DM, Verkhusha VV, Staroverov DB, Souslova EA, Lukyanov S, Lukyanov KA. Photoswitchable cyan fluorescent protein for protein tracking. Nat Biotechnol. 2004; 22 (11):1435-9. / pmid: 15502815
- Souslova EA, Chudakov DM. Photoswitchable cyan fluorescent protein as a FRET donor. Microsc Res Tech. 2006; 69 (3):207-9. / pmid: 16538627
- Zhang L, Gurskaya NG, Merzlyak EM, Staroverov DB, Mudrik NN, Samarkina ON, Vinokurov LM, Lukyanov S, Lukyanov KA. Method for real-time monitoring of protein degradation at the single cell level. Biotechniques. 2007; 42 (4):446, 448, 450. / pmid: 17489230
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