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Cyan-to-green photoswitchable fluorescent protein PS-CFP2
- Monomer, succesful perfomance in fusions
- Irreversible photoconversion from a cyan to a green fluorescent form
- High contrast of photoconversion
- High pH stability allowing labeling of acidic organelles
- Recommended for tracking cell, organelle, and protein movement, monitoring the protein turnover and superresolution imaging
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PS-CFP2 is an improved mutant of the photoswitchable monomeric fluorescent protein PS-CFP [Chudakov et al., 2004]. PS-CFP2 exhibits faster maturation and a brighter fluorescence both before and after photoswitching than its parental variant.
PS-CFP2 is capable of irreversible photoconversion from cyan to green fluorescent form in response to 405 nm light irradiation. It is recommended for real-time in vivo tracking movement of individual cells, organelles, and protein fractions [Chudakov et al., 2007]. It can also be applied for monitoring of the protein turnover at the single cell level [Zhang et al., 2007] and superresolution imaging by PALM [Shroff et al., 2007]. In addition, PS-CFP2 can be used as a routine cyan fluorescent tag (excitation maximum at 400 nm and emission maximum at 468 nm) at moderate excitation intensities and as a donor in FRET applications [Souslova and Chudakov, 2006].
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Related products| PRODUCT | CAT.# | DESCRIPTION | SIZE | PRICE | | The prices do not include delivery. The prices vary in different countries. Please contact your local distributor for exact prices and delivery information. |
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| PS-CFP2 expression/source vectors |
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| pPS-CFP2-C |
FP801 |
Mammalian expression vector encoding humanized PS-CFP2 and allowing its expression and generation of fusions to the PS-CFP2 C-terminus |
20 μg |
€ 400 |
| pPS-CFP2-N |
FP802 |
Mammalian expression vector encoding humanized PS-CFP2 and allowing its expression and generation of fusions to the PS-CFP2 N-terminus |
20 μg |
€ 400 |
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| Antibody against PS-CFP2 |
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Anti-Tag(CGY)FP
antibody |
AB121 |
Rabbit polyclonal antibody against TagCFP, TagGFP, TagGFP2, TagYFP, PS-CFP2, and EGFP |
100 μg |
€ 150 |
| AB122 |
200 μg |
€ 200 |
References:
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Chudakov DM, Lukyanov S, Lukyanov KA.
Tracking intracellular protein movements using photoswitchable fluorescent proteins PS-CFP2 and Dendra2.
Nat Protoc. 2007; 2 (8):2024-32. / pmid: 17703215
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Chudakov DM, Verkhusha VV, Staroverov DB, Souslova EA, Lukyanov S, Lukyanov KA.
Photoswitchable cyan fluorescent protein for protein tracking.
Nat Biotechnol. 2004; 22 (11):1435-9. / pmid: 15502815
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Shroff H, Galbraith CG, Galbraith JA, White H, Gillette J, Olenych S, Davidson MW, Betzig E.
Dual-color superresolution imaging of genetically expressed probes within individual adhesion complexes.
Proc Natl Acad Sci U S A. 2007; 104 (51):20308-13. / pmid: 18077327
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Souslova EA, Chudakov DM.
Photoswitchable cyan fluorescent protein as a FRET donor.
Microsc Res Tech. 2006; 69 (3):207-9. / pmid: 16538627
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Zhang L, Gurskaya NG, Merzlyak EM, Staroverov DB, Mudrik NN, Samarkina ON, Vinokurov LM, Lukyanov S, Lukyanov KA.
Method for real-time monitoring of protein degradation at the single cell level.
Biotechniques. 2007; 42 (4):446, 448, 450. / pmid: 17489230
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