|
Photoactivatable fluorescent proteins
- Light-induced high-contrast photoconversion
- Easy detection by flow cytometry or fluorescence microscopy
- No cofactors, substrates, or chemical staining required
- Suitability for long-term tracking of cellular events
Photoactivatable fluorescent proteins (PAFPs) represent a novel effective tool for monitoring cellular events. PAFPs change spectral properties in response to irradiation with specific light. This provides unique possibilities for the optical labelling and tracking living cells, organelles, and intracellular molecules in a spatio-temporal manner. Moreover, PAFPs open new possibilities to study cell physiology, in particular, they allow careful determination of protein half-life.
|
PS-CFP2
Monomeric cyan-to-green photoswitchable fluorescent protein:
- up to 2000-fold contrast of photoconversion
- high pH stability
|
KFP-Red
Kindling red fluorescent protein:
- fluorescence is induced by green laser irradiation
- can be kindled reversibly or irreversibly
|
|
Available proteins
| CHARACTERISTIC |
PS-CFP2 | KFP-Red |
* Brightness is a product of extinction coefficient and quantum yield, divided by 1000.
Ext.coeff. - extinction coefficient. |
|
|
|
|
|
| Fluorescence color | cyan / green |
NO / red |
| Excitation max (nm) | 400 / 490 |
580 / 580 |
| Emission max (nm) | 468 / 511 |
600 / 600 |
| Quantum yield | 0.2 / 0.23 |
<0.001 / 0.07 |
| Ext. coeff. (M-1cm-1) | 43 000 / 47 000 |
123 000 / 59 000 |
| Brightness* | 8.6 / 10.81 |
0 / 4.1 |
| Activating light | UV-violet (e.g. 405 nm) |
green (530-560 nm) |
| Calculated contrast, fold | up to 2000 |
35-70 |
| Structure | monomer |
tetramer |
| Cell toxicity | not observed |
not observed |
| Aggregation | no |
no |
| Maturation rate at 37°C | fast |
medium |
| Molecular weight (kDa) | 27 |
26 |
Discontinued proteins: Dendra2
|
