Photoactivatable fluorescent proteins

- Light-induced high-contrast photoconversion
- Easy detection by flow cytometry or fluorescence microscopy
- No cofactors, substrates, or chemical staining required
- Suitability for long-term tracking of cellular events

Photoactivatable fluorescent proteins (PAFPs) represent a novel effective tool for monitoring cellular events. PAFPs change spectral properties in response to irradiation with specific light. This provides unique possibilities for the optical labelling and tracking living cells, organelles, and intracellular molecules in a spatio-temporal manner. Moreover, PAFPs open new possibilities to study cell physiology, in particular, they allow careful determination of protein half-life.

PS-CFP2
Monomeric cyan-to-green photoswitchable fluorescent protein:
- up to 2000-fold contrast of photoconversion
- high pH stability

KFP-Red
Kindling red fluorescent protein:
- fluorescence is induced by green laser irradiation
- can be kindled reversibly or irreversibly

Available proteins
CHARACTERISTIC PS-CFP2KFP-Red
* Brightness is a product of extinction coefficient and quantum yield, divided by 1000.
Ext.coeff. - extinction coefficient.
Fluorescence colorcyan   /   green NO   /   red
Excitation max (nm)400   /   490 580   /   580
Emission max (nm)468   /   511 600   /   600
Quantum yield0.2   /   0.23 <0.001   /   0.07
Ext. coeff. (M-1cm-1)43 000   /   47 000 123 000   /   59 000
Brightness*8.6   /   10.81 0   /   4.1
Activating lightUV-violet (e.g. 405 nm) green (530-560 nm)
Calculated contrast, foldup to 2000 35-70
Structuremonomer tetramer
Cell toxicitynot observed not observed
Aggregationno no
Maturation rate at 37°Cfast medium
Molecular weight (kDa)27 26

Discontinued proteins:

Dendra2

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