Photoactivatable fluorescent proteins

- Light-induced high-contrast photoconversion
- Easy detection by flow cytometry or fluorescence microscopy
- No cofactors, substrates, or chemical staining required
- Suitability for long-term tracking of cellular events

Photoactivatable fluorescent proteins (PAFPs) represent a novel effective tool for monitoring cellular events. PAFPs change spectral properties in response to irradiation with specific light. This provides unique possibilities for the optical labelling and tracking living cells, organelles, and intracellular molecules in a spatio-temporal manner. Moreover, PAFPs open new possibilities to study cell physiology, in particular, they allow careful determination of protein half-life.

Available proteins


PS-CFP2
Monomeric cyan-to-green photoswitchable fluorescent protein:
- up to 2000-fold contrast of photoconversion
- high pH stability


Dendra2
Monomeric green-to-red photoswitchable fluorescent protein:
- up to 4000-fold contrast of photoconversion
- switched by UV and blue light


KFP-Red
Kindling red fluorescent protein:
- fluorescence is induced by green laser irradiation
- can be kindled reversibly or irreversibly

Related products
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The prices do not include delivery. The prices vary in different countries. Please contact your local distributor for exact prices and delivery information.
Expression/source vectors
pPS-CFP2-CFP801Mammalian expression vector encoding humanazied PS-CFP2 and allowing its expression and generation of fusions to the PS-CFP2 C-terminus20 μg€ 400.00
pPS-CFP2-NFP802Mammalian expression vector encoding humanazied PS-CFP2 and allowing its expression and generation of fusions to the PS-CFP2 N-terminus20 μg€ 400.00
pDendra2-CFP821Mammalian expression vector encoding humanazied Dendra2 and allowing its expression and generation of fusions to the Dendra2 C-terminus; supplemented with pDendra2-Fibrillarin vector for control experiments20 μg€ 400.00
pDendra2-NFP822Mammalian expression vector encoding humanazied Dendra2 and allowing its expression and generation of fusions to the Dendra2 N-terminus; supplemented with pDendra2-Fibrillarin vector for control experiments20 μg€ 400.00
pDendra2-BFP823Bacterial expression vector; source of the Dendra2 coding sequence20 μg€ 400.00
Gateway® Dendra2-At-C entry cloneFP824Gateway® entry clone for generation of fusions to the C-terminus of Arabidopsis-optimized Dendra2; transfer of Dendra2 or Dendra2-tagged fusion into a Gateway® destination vector20 μg€ 400.00
Gateway® Dendra2-At-N entry cloneFP825Gateway® entry clone for generation of fusions to the N-terminus of Arabidopsis-optimized Dendra2; transfer of Dendra2 or Dendra2-tagged fusion into a Gateway® destination vector20 μg€ 400.00
pKindling-Red-NFP301Mammalian expression vector encoding humanazied KFP-Red and allowing its expression and generation of fusions to the KFP-Red N-terminus20 μg€ 400.00
pKindling-Red-BFP302Bacterial expression vector; source of the KFP-Red coding sequence20 μg€ 400.00
pKindling-Red-mitoFP401Mammalian expression vector encoding humanazied KFP-Red targeted to mitochondria20 μg€ 400.00
Vector sets
Mito-tracker vector setFPM01Mammalian expression vectors for fluorescent labeling of mitochondria: pTurboGFP-mito, pPhi-Yellow-mito, and pKindling-Red-mito20 μg each€ 700.00
Recombinant proteins
rKFP-RedFP351Purified recombinant kindling red fluorescent protein100 μg€ 130.00
Antibodies
Anti-Tag(CGY)FP
antibody
AB121Rabbit polyclonal antibody against TagCFP, TagGFP, TagYFP, PS-CFP2, and EGFP100 μg€ 150.00
AB122200 μg€ 200.00
Anti-Tag(CGY)FP
antibody
AB121Rabbit polyclonal antibody against TagCFP, TagGFP, TagYFP, PS-CFP2, and EGFP100 μg€ 150.00
AB122200 μg€ 200.00
Anti-Dendra2
antibody
AB821Rabbit polyclonal antibody against Dendra2100 μg€ 150.00
AB822200 μg€ 200.00
Anti-Dendra2
antibody
AB821Rabbit polyclonal antibody against Dendra2100 μg€ 150.00
AB822200 μg€ 200.00
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