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Kindling red fluorescent protein KFP-Red
- Reversible or irreversible photoactivation
- Activated by green light that does not damage cells and tissues
- Quenching by blue light
- Recommended for tracking cells and cellular organelle movements
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KFP-Red (also referred to as KFP1) is a photoactivatable GFP-like protein generated on the basis of Anemonia sulcata chromoprotein, asFP595 [Lukyanov et al., 2000; Chudakov et al., 2003a; Chudakov et al., 2003b]. KFP-Red switches from a non-fluorescent to a red fluorescent form (with excitation/emission maxima at 580 nm and 600 nm, respectively) under the exposure to intense green light irradiation. A green light laser does not damage cells and tissues. Activated KFP-Red can be easily detected because its emission spectrum is beyond the region of cell autofluorescence.
KFP-Red can be used for in vivo monitoring cell and cellular organelle movement.
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Related products| PRODUCT | CAT.# | DESCRIPTION | SIZE | PRICE | | The prices do not include delivery. The prices vary in different countries. Please contact your local distributor for exact prices and delivery information. |
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| KFP-Red expression/source vectors |
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| pKindling-Red-N |
FP301 |
Mammalian expression vector encoding humanized KFP-Red and allowing its expression and generation of fusions to the KFP-Red N-terminus |
20 μg |
€ 400 |
| pKindling-Red-B |
FP302 |
Bacterial expression vector; source of the KFP-Red coding sequence |
20 μg |
€ 400 |
| pKindling-Red-mito |
FP401 |
Mammalian expression vector encoding humanized KFP-Red targeted to mitochondria |
20 μg |
€ 400 |
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| Vector set |
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| Mito-tracker |
FPM01 |
Mammalian expression vectors for fluorescent labeling of mitochondria: pTurboGFP-mito, pPhi-Yellow-mito, and pKindling-Red-mito |
20 μg each |
€ 700 |
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| Recombinant protein |
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| rKFP-Red |
FP351 |
Purified recombinant kindling red fluorescent protein |
100 μg |
€ 130 |
References:
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Chudakov DM, Belousov VV, Zaraisky AG, Novoselov VV, Staroverov DB, Zorov DB, Lukyanov S, Lukyanov KA.
Kindling fluorescent proteins for precise in vivo photolabeling.
Nat Biotechnol. 2003a; 21 (2):191-4. / pmid: 12524551
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Chudakov DM, Feofanov AV, Mudrik NN, Lukyanov S, Lukyanov KA.
Chromophore environment provides clue to "kindling fluorescent protein" riddle.
J Biol Chem. 2003b; 278 (9):7215-9. / pmid: 12496281
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Lukyanov KA, Fradkov AF, Gurskaya NG, Matz MV, Labas YA, Savitsky AP, Markelov ML, Zaraisky AG, Zhao X, Fang Y, Tan W, Lukyanov SA.
Natural animal coloration can Be determined by a nonfluorescent green fluorescent protein homolog.
J Biol Chem. 2000; 275 (34):25879-82. / pmid: 10852900
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