Dendra2


Green-to-red photoswitchable fluorescent protein Dendra2

- Monomer, successful performance in fusions
- Irreversible photoconversion from a green to a red fluorescent form
- High contrast of photoconversion
- Activated by UV-violet and blue light
- Matures at a wide range of temperatures
- Recommended for tracking cell, organelle, and protein movement, and for determination of protein half-life

Dendra2 is an improved version of a green-to-red photoswitchable fluorescent protein Dendra, derived from octocoral Dendronephthya sp. [Gurskaya et al., 2006]. Dendra2 exhibits faster maturation and brighter fluorescence both before and after photoswitching than that of Dendra.
Dendra2 is capable of irreversible photoconversion from a green to a red fluorescent form. Comparing with other available photoactivatable proteins, it provides a unique combination of advantageous properties including monomeric state suitable for protein labeling, high contrast photoconversion with fluorescence at the red spectral region, low-phototoxic activation with 488-nm light available on common confocal microscopes, high photostability of the photoconverted state, and efficient chromophore maturation at 37°C in mammalian cells. These properties make Dendra2 an ideal tool for real-time tracking protein dynamics (movement, degradation, etc.) and monitoring selective cell fate [Gurskaya et al., 2006; Zhang et al., 2007; Chudakov et al., 2007].

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Dendra2 expression/source vectors

pDendra2-CFP821Mammalian expression vector encoding humanazied Dendra2 and allowing its expression and generation of fusions to the Dendra2 C-terminus; supplemented with pDendra2-Fibrillarin vector for control experiments20 μg€ 400.00
pDendra2-NFP822Mammalian expression vector encoding humanazied Dendra2 and allowing its expression and generation of fusions to the Dendra2 N-terminus; supplemented with pDendra2-Fibrillarin vector for control experiments20 μg€ 400.00
pDendra2-BFP823Bacterial expression vector; source of the Dendra2 coding sequence20 μg€ 400.00
Gateway® Dendra2-At-C entry cloneFP824Gateway® entry clone for generation of fusions to the C-terminus of Arabidopsis-optimized Dendra2; transfer of Dendra2 or Dendra2-tagged fusion into a Gateway® destination vector20 μg€ 400.00
Gateway® Dendra2-At-N entry cloneFP825Gateway® entry clone for generation of fusions to the N-terminus of Arabidopsis-optimized Dendra2; transfer of Dendra2 or Dendra2-tagged fusion into a Gateway® destination vector20 μg€ 400.00


Antibody against Dendra2

Anti-Dendra2 antibodyAB821Rabbit polyclonal antibody against Dendra2100 μg€ 150.00
AB822200 μg€ 200.00

References:

  • Chudakov DM, Lukyanov S, Lukyanov KA. Tracking intracellular protein movements using photoswitchable fluorescent proteins PS-CFP2 and Dendra2. Nat Protoc. 2007; 2 (8):2024-32. / pmid: 17703215
  • Gurskaya NG, Verkhusha VV, Shcheglov AS, Staroverov DB, Chepurnykh TV, Fradkov AF, Lukyanov S, Lukyanov KA. Engineering of a monomeric green-to-red photoactivatable fluorescent protein induced by blue light. Nat Biotechnol. 2006; 24 (4):461-5. / pmid: 16550175
  • Zhang L, Gurskaya NG, Merzlyak EM, Staroverov DB, Mudrik NN, Samarkina ON, Vinokurov LM, Lukyanov S, Lukyanov KA. Method for real-time monitoring of protein degradation at the single cell level. Biotechniques. 2007; 42 (4):446, 448, 450. / pmid: 17489230
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