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    PA-TagRFP

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Photoactivatable red fluorescent protein PA-TagRFP

- Monomer, successful performance in fusions
- Non-fluorescent before photoactivation
- Irreversible photoactivation to a red fluorescent form by UV-violet light irradiation
- High brightness and photostability
- Recommended for monitoring of protein movement with super-resolution imaging

PA-TagRFP is a photoactivatable mutant of the bright monomeric red fluorescent protein TagRFP [Subach et al., 2010]. PA-TagRFP is capable of irreversible photoconversion from non-fluorescent to red fluorescent form (with excitation/emission maxima at 562 nm and 595 nm, respectively) in response to UV-violet light irradiation.

High brightness, photostability and monomeric nature of PA-TagRFP make it an excellent protein tag for both conventional microscopy and super-resolution PALM imaging techniques [Subach et al., 2010].

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PA-TagRFP expression/source vectors

pPA-TagRFP-C FP811 Mammalian expression vector encoding humanized PA-TagRFP and allowing its expression and generation of fusions to the PA-TagRFP C-terminus 20 μg € 400
pPA-TagRFP-N FP812 Mammalian expression vector encoding humanized PA-TagRFP and allowing its expression and generation of fusions to the PA-TagRFP N-terminus 20 μg € 400
pPA-TagRFP-actin FP813 Mammalian expression vector encoding humanized PA-TagRFP fused with human cytoplasmic β-actin 20 μg € 400
pPA-TagRFP-tubulin FP814 Mammalian expression vector encoding humanized PA-TagRFP fused with human α-tubulin 20 μg € 400
pPA-TagRFP-H2B FP815 Mammalian expression vector encoding humanized PA-TagRFP fused with human histone H2B 20 μg € 400


Antibody against PA-TagRFP

Anti-tRFP
antibody		 AB233 Rabbit polyclonal antibody against TurboRFP, TurboFP602, TurboFP635, TurboFP650, NirFP, TagBFP, TagRFP, TagFP635, mKate2 and PA-TagRFP 100 μg € 150
AB234 200 μg € 200

References:

  • Subach FV, Patterson GH, Renz M, Lippincott-Schwartz J, Verkhusha VV. Bright monomeric photoactivatable red fluorescent protein for two-color super-resolution sptPALM of live cells. J Am Chem Soc. 2010; 132 (18):6481-91. / pmid: 20394363
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