Recommended pairs for FRET applications
PAIR	DONOR	ACCEPTOR	CALCULATED FORSTER DISTANCE (Ro)	FRET EFFICIENCY (E)

TagBFP-TagGFP2	TagBFP ex/em max 402/457 nm	TagGFP2 ex/em max 483/506 nm	5.3 nm	0.57
TagGFP2-TagRFP	TagGFP2 ex/em max 483/506 nm	TagRFP ex/em max 555/584 nm	5.7 nm	0.50

		Spectral overlap for TagBFP-TagGFP2 and TagGFP2-TagRFP FRET pairs. Excitation (thin lines) and emission (thick lines) spectra of TagBFP (blue), TagGFP2 (green) and TagRFP (red) are shown individually. Spectral overlap is filled with gray.

Spectral overlap for TagBFP-TagGFP2 and TagGFP2-TagRFP FRET pairs.

Excitation (thin lines) and emission (thick lines) spectra of TagBFP (blue), TagGFP2 (green) and TagRFP (red) are shown individually. Spectral overlap is filled with gray.