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Licensing opportunities: duplex-specific nuclease

- Specific to double-stranded DNA
- Thermostable
- Inhibited by EDTA

Duplex-specific nuclease (DSN) is an enzyme purified from hepatopancreas of Kamchatka crab (Shagin et al., 2002). DSN shows a strong preference for cleaving double-stranded (ds) DNA and DNA in DNA-RNA hybrid duplexes, compared with single-stranded (ss) DNA and RNA. Moreover, the cleavage rate of short, perfectly matched DNA duplexes by this enzyme is considerably higher than that for nonperfectly matched duplexes of the same length. DSN finds use in various applications to isolate single-stranded DNA from complex nucleic acids, for example in cDNA normalization method (Zhulidov et al., 2004; Zhulidov et al., 2005; Bogdanova et al., 2008), for quantitative telomeric overhang determination (Zhao et al., 2008), and for SNP detection (Shagin et al., 2002).

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Evrogen technology embodied in DSN is available for commercial use through an adaptable licensing program. The licensing options may include development of novel products and applications.

Evaluation of the technologies is easily available by purchase of the related research use product. Please see "Notice to Purchaser" section for the product of your interest for details.

For license information please contact Evrogen by e-mail at license@evrogen.com.

Related publications:

  • Bogdanova EA, Shagin DA, Lukyanov SA. Normalization of full-length enriched cDNA. Mol Biosyst. 2008; 4 (3):205-12. / pmid: 18437263
  • Cheung F, Haas BJ, Goldberg SM, May GD, Xiao Y, Town CD. Sequencing Medicago truncatula expressed sequenced tags using 454 Life Sciences technology. BMC Genomics. 2006 Oct 24;7:272. / pmid: 17062153
  • Deng W, Zhu X, Skogerbo G, Zhao Y, Fu Z, Wang Y, He H, Cai L, Sun H, Liu C, Li B, Bai B, Wang J, Jia D, Sun S, He H, Cui Y, Wang Y, Bu D, Chen R. Organization of the Caenorhabditis elegans small non-coding transcriptome: genomic features, biogenesis, and expression. Genome Res. 2006 Jan;16(1):20-9. / pmid: 16344563
  • Du C, Ge B, Liu Z, Fu K, Chan WC, McKeithan TW. PCR-based generation of shRNA libraries from cDNAs. BMC Biotechnol. 2006 Jun 21;6:28. / pmid: 16790063
  • Shagin DA, Rebrikov DV, Kozhemyako VB, Altshuler IM, Shcheglov AS, Zhulidov PA, Bogdanova EA, Staroverov DB, Rasskazov VA, Lukyanov S. A novel method for SNP detection using a new duplex-specific nuclease from crab hepatopancreas. Genome Res. 2002; 12 (12):1935-42. / pmid: 12466298
  • Shcheglov A, Zhulidov P, Bogdanova E, Shagin D. Normalization of cDNA Libraries. In: Nucleic Acids Hybridization: Modern Applications. Buzdin, Anton; Lukyanov, Sergey (Eds.) ISBN: 978-1-4020-6039-7. 2007; 97-124. http://www.springerlink.com
  • Zhao Y, Hoshiyama H, Shay JW, Wright WE. Quantitative telomeric overhang determination using a double-strand specific nuclease. Nucleic Acids Res. 2008; 36 (3):e14. / pmid: 18073199
  • Zhulidov PA, Bogdanova EA, Shcheglov AS, Vagner LL, Khaspekov GL, Kozhemyako VB, Matz MV, Meleshkevitch E, Moroz LL, Lukyanov SA, Shagin DA. Simple cDNA normalization using kamchatka crab duplex-specific nuclease. Nucleic Acids Res. 2004; 32 (3):e37. / pmid: 14973331
  • Zhulidov PA, Bogdanova EA, Shcheglov AS, Shagina IA, Wagner LL, Khazpekov GL, Kozhemyako VV, Lukyanov SA, Shagin DA. A method for the preparation of normalized cDNA libraries enriched with full-length sequences. Bioorg Khim. 2005; 31 (2):186-94. http://www.springerlink.com / pmid: 15889793
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