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Licensing opportunities: duplex-specific nuclease
- Specific to double-stranded DNA
- Thermostable
- Inhibited by EDTA
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Duplex-specific nuclease (DSN) is an enzyme purified from hepatopancreas of Kamchatka crab (Shagin et al., 2002). DSN shows a strong preference for cleaving double-stranded (ds) DNA and DNA in DNA-RNA hybrid duplexes, compared with single-stranded (ss) DNA and RNA. Moreover, the cleavage rate of short, perfectly matched DNA duplexes by this enzyme is considerably higher than that for nonperfectly matched duplexes of the same length.
DSN finds use in various applications to isolate single-stranded DNA from complex nucleic acids, for example in cDNA normalization method (Zhulidov et al., 2004, 2005; Bogdanova et al., 2008), for quantitative telomeric overhang determination (Zhao et al., 2007), and for SNP detection (Shagin et al., 2002).
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Evrogen technology embodied in DSN is available for commercial use through an adaptable licensing program. The licensing options may include development of novel products and applications.
Evaluation of the technologies is easily available by purchase of the related research use product. Please see “Notice to Purchaser” section of the product of your interest Web page for details.
For license information please contact Evrogen by e-mail at license@evrogen.com.
Related publications:
- Bogdanova E.A., Shagin D.A. and Lukyanov S.A. (2008) Normalization of full-length enriched cDNA. Mol. BioSyst., DOI: 10.1039/b715110c
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Cheung F., Haas B.J., Goldberg S.M.,May G.D., Xiao Y., and Town C. D. Sequencing Medicago truncatula expressed sequenced tags using 454 Life Sciences technology, BMC Genomics, 2006, 7, 272.
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Deng W., Zhu X., Skogerbo G., Zhao Y., Fu Z., Wang Y., He H., Cai L., Sun H., Liu C., Li B., Bai B., Wang J., etc. Organization of the Caenorhabditis elegans small non-coding transcriptome, genomic features, biogenesis, and expression, Genome Res., 2006, 16(1), 20-29.
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Du C., Ge B., Liu Z., Fu K., Chan W.C., and McKeithan T.W. PCR-based generation of shRNA libraries from cDNAs, BMC Biotechnol., 2006, 6, 28.
- Shagin D.A., Rebrikov D.V., Kozhemyako V.B., Altshuler I.M., Shcheglov A.S., Zhulidov P.A., Bogdanova E.A., Staroverov D.B., Rasskazov V.A., Lukyanov S. (2002) A novel method for SNP detection using a new duplex-specific nuclease from crab hepatopancreas. Genome Res. 12, 1935-1942.
PMID: 12466298
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Shcheglov A.S., Zhulidov A.S. , Bogdanova E.A. and Shagin D.A. Normalization of cDNA Libraries. In, Nucleic Acids Hybridization Modern Applications. (eds. A. Buzdin and S. Lukyanov), 2007, 97-124, Springer Netherlands.
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Zhao Y., Hoshiyama H, Shay J.W. and Wright W.E. uantitative telomeric overhang determination using a double-strand specific nuclease
Nucleic Acids Research, 2007, doi:10.1093/nar/gkm1063.
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Zhulidov P.A., Bogdanova E.A., Shcheglov A.S., Vagner L.L., Khaspekov G.L., Kozhemyako V.B., Matz M.V., Meleshkevitch E., Moroz L.L., Lukyanov S.A., Shagin D.A. (2004) Simple cDNA normalization using kamchatka crab duplex-specific nuclease. Nucleic Acid Res., 32: e37.
PMID: 14973331
- Zhulidov P.A., Bogdanova E.A., Shcheglov A.S., Shagina I.A., Wagner L.L., Khaspekov G.L., Kozhemyako V.B., Lukyanov S.A., Shagin,D.A. (2005) A method for the preparation of normalized cDNA libraries enriched with full length sequences. Russian Journal of Bioorganic Chemistry 31 (2):170 177.
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