Trimmer and Trimmer-Direct cDNA normalization kits

cat.# NK001; NK002

- Acceleration of transcriptome analysis, functional screening, and rare gene discovery
- Rapid and reliable way to remove repeated transcripts from cDNA library
- Equalization of full-length-enriched cDNA before library cloning
- Simple procedure, no physical separation steps

Evrogen offers cDNA normalization kits designed to generate full-length-enriched double stranded (ds) cDNA with equalized concentrations of different transcripts. Both total RNA and poly(A+) RNA can be used for cDNA preparation and subsequent normalization. Depending on your particular needs, you can select Trimmer or Trimmer-Direct kit wherein the first allows preparation of normalized cDNA ready for nondirectional cloning and the second – for directional cloning of cDNA library. Alternatively, DSN-normalized cDNA can be used for 454 sequencing.

Starting materials

Trimmer kit allows normalization of cDNA flanked by the same adapter sequences at the both 3'- and 5'- ends. Starting cDNA can be prepared using one of the following cDNA synthesis kits:

- Mint cDNA synthesis kit (Evrogen Cat.# SK001)
- Mint-Universal cDNA synthesis kit, Protocol-I (Evrogen Cat.# SK002)
- SMART^TM PCR cDNA synthesis kit (Clontech Cat.# 634902).

Trimmer-Direct kit is designed for normalization of cDNA flanked by the different adapter sequences at the 3'- and 5'- ends. Starting cDNA must be prepared using one of the following cDNA synthesis kits:

- Mint-Universal cDNA synthesis kit, Protocol-II (Evrogen Cat.# SK002)
- SMART^TM cDNA Library Construction kit (Clontech Cat.# 634901)*
- Creator^TM SMART^TM cDNA Library Construction kit (Clontech Cat.# 634903)*.

Typical cDNA normalization result. (A) Agarose/EtBr gel-electrophoresis of non-normalized (1) and normalized (2) human cDNA samples; (B, C) concentration of abundant transcripts in these samples revealed by Virtual Northern blot. GPDH - glyceraldehyde-3-phosphate dehydrogenase; UBC - ubiquitin C; M, 1-kb DNA size markers (SibEnzyme); embr. - embryonic; sm.intest. - small intestine.

Available products

Product	Cat.#	Description	Size	Price	User Manual (pdf)
Trimmer kit	NK001	Each package comprises a detailed instruction and reagents for cDNA normalization	10 rxn	€ 600.00
Trimmer-Direct kit	NK002	Each package comprises a detailed instruction and reagents for cDNA normalization	10 rxn	€ 750.00
The prices do not include delivery. The prices vary in different countries. Please contact your local distributor for exact prices and delivery information.

Storage: Lyophilized DSN enzyme should be stored at +4°C, other components should be stored at -20°C