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    KillerRed

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References citing KillerRed:
  • Vegh RB, Solntsev KM, Kuimova MK, Cho S, Liang Y, Loo BL, Tolbert LM, Bommarius AS.
    Reactive oxygen species in photochemistry of the red fluorescent protein "Killer Red".
    Chem Commun (Camb). 2011 May 7;47(17):4887-9
    pmid: 21359336
     
  • Liu X, Shi R, Zou D, Li Z, Liu X, Chen Y, Yang X, Zhou Y, Zheng D.
    Positive selection vector using the KillerRed gene.
    Anal Biochem. 2011 May 1;412(1):120-2
    pmid: 21284929
  1. Constructs used: pKillerRed-B vector, Evrogen.
  2. Expression system: E. coli.
  3. Detection system: KillerRed-mediated killing of E. coli by irradiation with white light for 20-60 min (medical surgical light, photon flux 1550 μmol/m2s).
  • Korzh V, Teh C, Kondrychyn I, Chudakov DM, Lukyanov S.
    Visualizing compound transgenic zebrafish in development: a tale of green fluorescent protein and KillerRed.
    Zebrafish. 2011 Mar;8(1):23-9
    pmid: 21348774
     
  • Waldeck W, Mueller G, Wiessler M, Tóth K, Braun K.
    Positioning effects of KillerRed inside of cells correlate with DNA strand breaks after activation with visible light.
    Int J Med Sci. 2011 Jan 21;8(2):97-105
    pmid: 21278894
  1. Constructs used: expression vectors encoding fusions of KillerRed with histone H2A and lamin B1 (constructed based on the pKillerRed-C and pKillerRed-N vectors, Evrogen).
  2. Expression system: human prostate cancer cell line DU145 transfected with the help of TurboFect reagent.
  3. Detection system: activation of KillerRed by illumination with visible light (0-60 min).
  • Lee A, Mathuru AS, Teh C, Kibat C, Korzh V, Penney TB, Jesuthasan S.
    The habenula prevents helpless behavior in larval zebrafish.
    Curr Biol. 2010 Dec 21;20(24):2211-6
    pmid: 21145744
     
  • Destaing O, Planus E, Bouvard D, Oddou C, Badowski C, Bossy V, Raducanu A, Fourcade B, Albiges-Rizo C, Block MR.
    beta1A integrin is a master regulator of invadosome organization and function.
    Mol Biol Cell. 2010 Dec;21(23):4108-19
    pmid: 20926684
     
  • Teh C, Chudakov DM, Poon KL, Mamedov IZ, Sek JY, Shidlovsky K, Lukyanov S, Korzh V.
    Optogenetic in vivo cell manipulation in KillerRed-expressing zebrafish transgenics.
    BMC Dev Biol. 2010 Nov 2;10:110
    pmid: 21040591
  1. Constructs used: membrane-targeted KillerRed expression vector.
  2. Expression system: transgenic zebrafish embryos.
  3. Detection system: Axiovert 200M upright microscope; activation of KillerRed with green light (UV filter set 15, BP 546/12nm, mercury arc lamp).
  • Del Bene F, Wyart C, Robles E, Tran A, Looger L, Scott EK, Isacoff EY, Baier H.
    Filtering of visual information in the tectum by an identified neural circuit.
    Science. 2010 Oct 29;330(6004):669-73
    pmid: 21030657
     
  • Lukyanov KA, Serebrovskaya EO, Lukyanov S, Chudakov DM.
    Fluorescent proteins as light-inducible photochemical partners.
    Photochem Photobiol Sci. 2010 Oct 28;9(10):1301-6
    pmid: 20672171
     
  • Roy A, Carpentier P, Bourgeois D, Field M.
    Diffusion pathways of oxygen species in the phototoxic fluorescent protein KillerRed.
    Photochem Photobiol Sci. 2010 Oct 28;9(10):1342-50
    pmid: 20820672
     
  • Liu X, Liu X, Zhou Y, Zou D, Shi R, Li Z, Zheng D.
    T vector bearing KillerRed protein marker for red/white cloning screening.
    Anal Biochem. 2010 Oct 15;405(2):272-4
    pmid: 20599648
  1. Constructs used: pKillerRed-B vector, Evrogen.
  2. Expression system: E. coli.
  3. Detection system: detecton of red fluorescents from KillerRed under fluorescent microscope with 510-560 nm excitation filter.
  • Butler MC, Itotia PN, Sullivan JM.
    A high-throughput biophotonics instrument to screen for novel ocular photosensitizing therapeutic agents.
    Invest Ophthalmol Vis Sci. 2010 May;51(5):2705-20
    pmid: 19834043
  1. Constructs used: KillerRed expressing vector.
  2. Expression system: stably transfected HEK 293 cells.
  3. Detection system: activation of KillerRed with LED at 575nm.
  • Waldeck W, Mueller G, Wiessler M, Brom M, Tóth K, Braun K.
    Autofluorescent proteins as photosensitizer in eukaryontes.
    Int J Med Sci. 2009 Dec 1;6(6):365-73
    pmid: 19960122
  1. Constructs used: pKillerRed-mem vector, Evrogen; expression vectors encoding fusions of KillerRed with histone H2A and Lamin B1 (constructed based on the pKillerRed-C and pKillerRed-N vectors, Evrogen).
  2. Expression system: stably transfected HeLa and DU145 cell lines (transfection with FuGENE reagent and selection with 500ug/ml of G418).
  3. Detection system: activation of KillerRed by illumination of cells in quartz cuvettes under one full-spectrum sunlight bulb with 32 Watt in a distance of 1 cm for 15-180 min.
  • Pletnev S, Gurskaya NG, Pletneva NV, Lukyanov KA, Chudakov DM, Martynov VI, Popov VO, Kovalchuk MV, Wlodawer A, Dauter Z, Pletnev V.
    Structural basis for phototoxicity of the genetically encoded photosensitizer KillerRed.
    J Biol Chem. 2009 Nov 13;284(46):32028-39
    pmid: 19737938
     
  • Carpentier P, Violot S, Blanchoin L, Bourgeois D.
    Structural basis for the phototoxicity of the fluorescent protein KillerRed.
    FEBS Lett. 2009 Sep 3;583(17):2839-42
    pmid: 19646983
     
  • Serebrovskaya EO, Edelweiss EF, Stremovskiy OA, Lukyanov KA, Chudakov DM, Deyev SM.
    Targeting cancer cells by using an antireceptor antibody-photosensitizer fusion protein.
    Proc Natl Acad Sci U S A. 2009 Jun 9;106(23):9221-5
    pmid: 19458251
  1. Constructs used: recombinant protein composed of D5scFV fragment (4D5 is an antibody – Herceptin – against Her2) fused to the KillerRed N-terminus.
  2. Detection system: used SKOV-3 cell line (human ovarian carcinoma) to demonstrate binding of the fusion construct to cell surface by FACS and ELISA. Also demonstrated phototoxicity effects of the fusion construct on SKOV-3 cells after inc ubation for 1 hour at 4C, followed by white light irradiation (1W/cm2 for 10 min).
  • Samarkina ON, Popova AG, Gvozdik EY, Chkalina AV, Zvyagin IV, Rylova YV, Rudenko NV, Lusta KA, Kelmanson IV, Gorokhovatsky AY, Vinokurov LM.
    Universal and rapid method for purification of GFP-like proteins by the ethanol extraction.
    Protein Expr Purif. 2009 May;65(1):108-13
    pmid: 19084068
     
  • Mocz G.
    Fluorescent proteins and their use in marine biosciences, biotechnology, and proteomics.
    Mar Biotechnol (NY). 2007 May-Jun;9(3):305-28
    pmid: 17372780
     
  • Remington SJ.
    Fluorescent proteins: maturation, photochemistry and photophysics.
    Curr Opin Struct Biol. 2006 Dec;16(6):714-21
    pmid: 17064887
     
  • Wolff M, Wiedenmann J, Nienhaus GU, Valler M, Heilker R.
    Novel fluorescent proteins for high-content screening.
    Drug Discov Today. 2006 Dec;11(23-24):1054-60
    pmid: 17129823
     
  • Giepmans BN, Adams SR, Ellisman MH, Tsien RY.
    The fluorescent toolbox for assessing protein location and function.
    Science. 2006 Apr 14;312(5771):217-24
    pmid: 16614209
     
  • Bulina ME, Chudakov DM, Britanova OV, Yanushevich YG, Staroverov DB, Chepurnykh TV, Merzlyak EM, Shkrob MA, Lukyanov S, Lukyanov KA.
    A genetically encoded photosensitizer.
    Nat Biotechnol. 2006 Jan;24(1):95-9
    pmid: 16369538
  1. Constructs used: vectors expressing KillerRed, mitochondria-targeted KillerRed, KillerRed fused with PH domain of phospholipase C.
  2. Expression system: E. coli, transiently transfected 293T and B16 cell lines.
  3. Detection system: activation of KillerRed by ilumination of cells (535-575nm excitation filter).
  • Bulina ME, Lukyanov KA, Britanova OV, Onichtchouk D, Lukyanov S, Chudakov DM.
    Chromophore-assisted light inactivation (CALI) using the phototoxic fluorescent protein KillerRed.
    Nat Protoc. 2006;1(2):947-53
    pmid: 17406328
  1. Constructs used: vectors expressing membrane-targeted or mitochondria-targeted KillerRed.
  2. Expression system: mammalian cell lines.
  3. Detection system: activation of KillerRed with green light. Olympus CK40 microscope (40xobjective, mercury lamp, 535-575nm exc, 3.3W/cm2) or Leica DM IRE2 (63xobj, mercury lamp, 515-560nm, 7W/cm2). Phototoxicity is associated with photobleaching.

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