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RESEARCH PRODUCTS/Genetically-encoded fluorescent biosensors/HyPer

pHyPer-Cyto

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pHyPer-Cyto vector

cat.# FP941

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

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vector information:

Product	Cat.#	Size	Price
pHyPer-Cyto	FP941	20 μg	€ 600.00
The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information. The product is not available in certain countries. Please contact Evrogen or your local distributor before ordering.

Vector type	mammalian expression vector
Reporter	HyPer
Reporter codon usage	mammalian / E. coli
Promoter for HyPer	P_{CMV IE}
Host cells	mammalian
Selection	prokaryotic - kanamycin eukaryotic - neomycin (G418)
Replication	prokaryotic - pUC ori eukaryotic - SV40 ori
Use	HyPer expression in mammalian cells under the control of CMV promoter; source of HyPer coding sequence

Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
Hyper
Kozak consensus translation initiation site: 596-606
Start codon (ATG): 603-605
Stop codon: 2037-2039
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 2238-2243 & 2267-2272
mRNA 3' ends: 2276 & 2288
f1 single-strand DNA origin: 2335-2790
Eukaryotic promoter for expression of Kan^r gene
-35 region: 2852-2857
-10 region: 2875-2880
Transcription start point: 2887
SV40 origin of replication: 3131-3266
SV40 early promoter
Enhancer (72-bp tandem repeats): 2964-3035 & 3036-3107
21-bp repeats: 3111-3131, 3132-3152 & 3154-3174
Early promoter element: 3187-3193
Major transcription start points: 3183, 3221, 3227 & 3232
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 3315-3317
Stop codon: 4107-4109
G->A mutation to remove Pst I site: 3497
C->A (Arg to Ser) mutation to remove BssH II site: 3843
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 4345-4350 & 4358-4363
pUC plasmid replication origin: 4694-5337

Notice to Purchaser:

The HyPer-related materials (also referred to as "Products") are intended for research use only. These products are covered by Evrogen Patents and/or Patent applications pending.

The CMV promoter is covered under U.S. Patents 5,168,062 and 5,385,839, and its use is permitted for research purposes only. Any other use of the CMV promoter requires a license from the University of Iowa Research Foundation, 214 Technology Innovation Center, Iowa City, IA 52242.

MATERIAL SAFETY DATA SHEET INFORMATION

To the best of our knowledge, these products do not require a Material Safety Data Sheet. However, all the properties of these products (and, if applicable, each of their components) have not been thoroughly investigated. Therefore, we recommend that you use gloves and eye protection, and wear a laboratory coat when working with these products.


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