|
References citing Case12:
|
-
Chang KT, Niescier RF, Min KT.
Mitochondrial matrix Ca2+ as an intrinsic signal regulating mitochondrial motility in axons.
Proc Natl Acad Sci U S A. 2011 Sep 13;108(37):15456-61
pmid: 21876166
|
|
-
Figueiredo M, Lane S, Tang F, Liu BH, Hewinson J, Marina N, Kasymov V, Souslova EA, Chudakov DM, Gourine AV, Teschemacher AG, Kasparov S.
Optogenetic experimentation on astrocytes.
Exp Physiol. 2011 Jan;96(1):40-50
pmid: 21041318
|
|
-
Guo F, Liu B, Tang F, Lane S, Souslova EA, Chudakov DM, Paton JF, Kasparov S.
Astroglia are a possible cellular substrate of angiotensin(1-7) effects in the rostral ventrolateral medulla.
Cardiovasc Res. 2010 Aug 1;87(3):578-84
pmid: 20200044
|
-
Constructs used: adenoviral vector carrying Case12 coding sequence (subcloned from pCase12-cyto vector, Evrogen).
-
Expression system: primary cultures of rat neurones and astrocytes transfected with Case12 expressing adenoviral vectors.
-
Detection system: Leica confocal SP1 scanner (excitation with 488nm laser line, minimum laser intensity).
|
-
Gourine AV, Kasymov V, Marina N, Tang F, Figueiredo MF, Lane S, Teschemacher AG, Spyer KM, Deisseroth K, Kasparov S.
Astrocytes control breathing through pH-dependent release of ATP.
Science. 2010 Jul 30;329(5991):571-5
pmid: 20647426
|
-
Constructs used: adenoviral vector carrying Case12 coding sequence (subcloned from pCase12-cyto vector, Evrogen).
-
Expression system: primary cultures of rat astrocytes transfected with Case12 expressing adenoviral vector.
-
Detection system: Leica fluorescence microscope and MiCam02 high-resolution camera (excitation with 488 nm laser line, at 0.5–0.7% of laser output, emission collected using a bandpass filter 505–550nm).
|
-
Zhu X, Caplan J, Mamillapalli P, Czymmek K, Dinesh-Kumar SP.
Function of endoplasmic reticulum calcium ATPase in innate immunity-mediated programmed cell death.
EMBO J. 2010 Mar 3;29(5):1007-18
pmid: 20075858
|
-
Constructs used: plant cells expression vector carrying Case12 coding sequence (subcloned from pCase12-cyto vector, Evrogen).
-
Expression system: Case12 transiently expressed in N. benthamiana leaves using agroinfiltration.
-
Detection system: Zeiss Axiovert 200M light microscope equipped with a LSM 510 NLO confocal microscope.
|
-
Hynes J, O'Riordan TC, Zhdanov AV, Uray G, Will Y, Papkovsky DB.
In vitro analysis of cell metabolism using a long-decay pH-sensitive lanthanide probe and extracellular acidification assay.
Anal Biochem. 2009 Jul 1;390(1):21-8
pmid: 19379702
|
-
Constructs used: pCase12-mito vector, Evrogen (mitochondria-trageted Case12).
-
Expression system: PC12 cell line (rat pheochromocytoma) transiently transfected using Lipofectamine reagent.
-
Detection system: Olympus FV 1000 confocal microscope (Case12 excitation at 488nm, 1.5% of laser power, emission detected at 500-550 nm).
|
-
Guo F, Souslova E, Chudakov D, Paton JFR, Kasparov S.
Cellular substrates for angiotensin1-7 (Ang1-7) action in the rostral ventro-lateral medulla (RVLM) of the normotentsive and spontaneously hypertensive rat (SHR).
FASEB J. 2009; 23(1_MeetingAbstracts):958.3
|
|
-
Souslova EA, Chudakov DM.
Genetically encoded intracellular sensors based on fluorescent proteins.
Biochemistry (Mosc). 2007 Jul;72(7):683-97
pmid: 17680759
|
|
-
Souslova EA, Belousov VV, Lock J, Stromblad S, Kasparov S, Bolshakov AP, Pinelis VG, Labas YA, Lukyanov S, Mayr LM, Chudakov DM.
Single fluorescent protein-based Ca2+ sensors with increased dynamic range.
BMC Biotechnol. 2007 Jun 29;7:37
pmid: 17603870
|
-
Constructs used: Case12 expressing plasmid.
-
Expression system: M21 (human melanoma) and HeLa cell lines transiently transfected with Case12 expressing plasmid using FuGENE reagent; primary rat cortical neurons transiently transfected with Lipofectamine 2000 reagent.
-
Detection system: Zeiss LSM 510 confocal microscope (Case12 excitation with 488nm laser line, emission collected at 500-540 nm).
|
Find more:
|
