Green-to-red photoswitchable fluorescent protein Dendra2

- Monomer, successful performance in fusions
- Irreversible photoconversion from a green to a red fluorescent form
- High contrast of photoconversion
- Activated by UV-violet and blue light
- Matures at a wide range of temperatures
- Recommended for tracking cell, organelle, and protein movement, and for determination of protein half-life

Main properties

Normalized excitation (thin line) and emission (thick line) for non-activated (green) and activated (red) spectra. Download Dendra2 spectra (xls)

CHARACTERISTIC before  /  after photoactivation *Brightness is a product of extinction coefficient and quantum yield, divided by 1000. Fluorescence color green   /   red Excitation maximum, nm 490   /   553 Emission maximum, nm 507   /   573 Quantum yield 0.50   /   0.55 Extinction coefficient, M-1cm-1 45 000   /   35 000 Brightness* 22.5   /   19.25 pKa 6.6   /   6.9 Activating light UV-violet (e.g. 405 nm) or blue (e.g. 488 nm) Calculated contrast, fold up to 4000 Structure monomer Cell toxicity not observed Aggregation no Maturation rate at 37°C fast Molecular weight, kDa 26 Polypeptide length, aa 238 Main advantages Bright monomeric high-contrast irreversibly photoswitchable tag suitable for protein tracking; capable for switching under blue light