Normalized excitation (thin line) and emission (thick line) spectra for activated PA-TagRFP.
Download PA-TagRFP spectra (xls)
| CHARACTERISTIC | before / after photoactivation |
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* Brightness is a product of extinction coefficient and quantum yield, divided by 1000.
| Fluorescence color | NO / red | Excitation maximum, nm | - / 562 | Emission maximum, nm | - / 595 | Quantum yield | nd / 0.38 | Extinction coefficient, M-1cm-1 | nd / 66 000 | Brightness* | 0 / 25.1 | pKa | nd / 5.3 | Activating light | UV-violet (e.g. 390-420 nm) | Calculated contrast, fold | ~540 | Structure | monomer | Cell toxicity | not observed | Aggregation | no | Maturation rate at 37°C | fast | Molecular weight, kDa | 27 | Polypeptide length, aa | 237 |
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