RESEARCH PRODUCTS/Nucleic acid research products:

    cDNA normalization kits

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Trimmer and Trimmer-Direct cDNA normalization kits

cat.# NK001; NK002

- Acceleration of transcriptome analysis, functional screening, and rare gene discovery
- Rapid and reliable way to remove repeated transcripts from cDNA library
- Equalization of full-length-enriched cDNA before library cloning
- Simple procedure, no physical separation steps

Evrogen offers cDNA normalization kits designed to generate full-length-enriched double stranded (ds) cDNA with equalized concentrations of different transcripts. Both total RNA and poly(A)+ RNA can be used for cDNA preparation and subsequent normalization.
Depending on your particular needs, you can select Trimmer or Trimmer-Direct kit wherein the first allows preparation of normalized cDNA ready for nondirectional cloning and the second – for directional cloning of cDNA library. Alternatively, DSN-normalized cDNA can be used for 454 sequencing.
Important note regarding 454 sequencing.

Available products

PRODUCTCAT.#DESCRIPTIONSIZEPRICEUSER MANUAL (PDF)
The prices do not include delivery. The prices vary in different countries. Please contact your local distributor for exact prices and delivery information.
Trimmer kit NK001 Allows normalization of cDNA for subsequent non-directional cloning 10 rxn € 600
Trimmer-Direct kit NK002 Allows normalization of cDNA for subsequent directional cloning 10 rxn € 750

Storage: Lyophilized DSN enzyme should be stored at +4°C, other components should be stored at -20°C

Notice to Purchaser:

Evrogen Nucleic Acid-related Products (the Products) are intended for research use only. The Products are covered by U.S. Pat. # 7,435,794 and other Evrogen Patents and/or Patent applications pending. By use of these Products, you accept the terms and conditions of the applicable Limited Use Label License #002.

MATERIAL SAFETY DATA SHEET INFORMATION

To the best of our knowledge, these products do not require a Material Safety Data Sheet. However, all the properties of these products (and, if applicable, each of their components) have not been thoroughly investigated. Therefore, we recommend that you use gloves and eye protection, and wear a laboratory coat when working with these products.

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