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    pTagCFP-actin

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pTagCFP-actin vector

cat.# FP114

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

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vector information:
ProductCat.#SizePrice
pTagCFP-actinFP11420 μg€ 400.00
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Vector typemammalian expression vector
ReporterTagCFP
Reporter codon usagemammalian
Promoter for TagCFPPCMV IE
Host cellsmammalian
Selectionprokaryotic - kanamycin
eukaryotic - neomycin (G418)
Replicationprokaryotic - pUC ori
eukaryotic - SV40 ori
Use cyan fluorescent labeling of β-actin filaments

Location of features

PCMV IE: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
Kozak consensus translation initiation site: 606-616
TagCFP
Start codon (ATG): 613-615
Last amino acid in TagCFP: 1324-1326
Beta-Actin: 1348-2475
Stop codon: 2473-2475
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 2636-2641 & 2665-2670
mRNA 3' ends: 2674 & 2686
f1 single-strand DNA origin: 2733-3188
Eukaryotic promoter for expression of Kanr gene
-35 region: 3250-3255
-10 region: 3273-3278
Transcription start point: 3285
SV40 origin of replication: 3529-3664
SV40 early promoter
Enhancer (72-bp tandem repeats): 3362-3433 & 3434-3505
21-bp repeats: 3509-3529, 3530-3550 & 3552-3572
Early promoter element: 3585-3591
Major transcription start points: 3581, 3619, 3625 & 3630
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 3713-3715
Stop codon: 4505-4507
G->A mutation to remove Pst I site: 3895
C->A (Arg to Ser) mutation to remove BssH II site: 4241
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 4743-4748 & 4756-4761
pUC plasmid replication origin: 5092-5735


Notice to Purchaser:

Evrogen Fluorescent Protein Products (the Products) are intended for research use only and covered by Evrogen Patents and/or Patent applications pending. By use of these products, you accept the terms and conditions of the applicable Limited Use Label License.

The CMV promoter is covered under U.S. Patents 5,168,062 and 5,385,839, and its use is permitted for research purposes only. Any other use of the CMV promoter requires a license from the University of Iowa Research Foundation, 214 Technology Innovation Center, Iowa City, IA 52242.

MATERIAL SAFETY DATA SHEET INFORMATION

To the best of our knowledge, these products do not require a Material Safety Data Sheet. However, all the properties of these products (and, if applicable, each of their components) have not been thoroughly investigated. Therefore, we recommend that you use gloves and eye protection, and wear a laboratory coat when working with these products.

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