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RESEARCH PRODUCTS/Basic fluorescent proteins/TurboColors/TurboRFP

pTurboRFP-B

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pTurboRFP-B vector

cat.# FP233

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

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vector information:

Product	Cat.#	Size	Price
pTurboRFP-B	FP233	20 μg	€ 400.00
The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information.

Vector type	bacterial expression vector
Reporter	TurboRFP
Reporter codon usage	mammalian
Promoter for TurboRFP	T5 promoter/lac operator
Host cells	prokaryotic
Selection	ampicilin
Replication	ColE1 ori
Use	Source of the mammalian TurboRFP coding sequence; TurboRFP expression in bacterial cells

5'-Region
				TurboRFP


			BamH I
RBS	ATG.	AGA.GGA.TCG.	GGA.TCC.	ATG.AGC.GAG...

3'-Region
	Stop


		Hind III
...GGG.CAC.AGA.	TG	A.	AGC.TT

Vector description

pTurboRFP-B is a a prokaryotic expression vector encoding red (orange) fluorescent protein TurboRFP (see protein description). Reporter codon usage is optimized for high expression in mammalian cells (humanized) [Haas et al., 1996].

The vector is primarily intended as a source of TurboRFP coding sequence. Flanking restriction sites are convenient for TurboRFP gene excision and its further insertion into other expression vectors of choice. Alternatively, TurboRFP coding sequence can be amplified by PCR.

Note: The plasmid DNA was isolated from dam⁺-methylated E.coli. Therefore some restriction sites are blocked by methylation. If you wish to digest the vector using such sites you will need to transform the vector into a dam^- host and make fresh DNA.

The vector can be also used for TurboRFP expression in prokaryotes under the control of T5 promoter/lac operator. The vector backbone contains ColE1 origin of replication and ampicillin resistance gene for propagation and selection in E. coli.

Location of features

T5 promoter/lac operator element: 7-87
T5 transcription start: 61
TurboRFP coding sequence: 133-828
Lambda t0 transcriptional termination region: 849-943
rrnB T1 transcriptional termination region: 1705-1803
ColE1 origin of replication: 2279
beta-lactamase coding sequence: 3897-3037

References:

Haas J, Park EC, Seed B. Codon usage limitation in the expression of HIV-1 envelope glycoprotein. Curr Biol. 1996; 6 (3):315-24. / pmid: 8805248

Notice to Purchaser:

Evrogen FP-related products are intended for research use only and covered by Evrogen Patents and/or Patent applications pending. By use of these products, you accept the terms and conditions of the applicable Limited Use Label License.

MATERIAL SAFETY DATA SHEET INFORMATION

To the best of our knowledge, these products do not require a Material Safety Data Sheet. However, all the properties of these products (and, if applicable, each of their components) have not been thoroughly investigated. Therefore, we recommend that you use gloves and eye protection, and wear a laboratory coat when working with these products.


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