Hydrogen peroxide sensor HyPer

- Ratiometric measurement of intracellular hydrogen peroxide - Direct expression in cells
- No exogenous chemical compounds or cofactors required
- High selectivity and sensitivity
- No artifactual ROS generation
- Recommended for monitoring hydrogen peroxide production inside living cells under various physiological and pathological conditions

Main properties

HyPer excitation (thin line) and emission (thick line) spectra.	CHARACTERISTIC   Emission maximum, nm 516 Excitation maximum, nm 420 and 500 Fluorescence color green Polypeptide length, aa 478 Molecular weight, kDa 52 Specificity H2O2 Sensitivity submicromolar H2O2 concentrations pKa 4.7 Structure monomer Aggregation no Maturation rate at 37°C fast