Hydrogen peroxide sensor HyPer

- Ratiometric measurement of intracellular hydrogen peroxide - Direct expression in cells
- No exogenous chemical compounds or cofactors required
- High selectivity and sensitivity
- No artifactual ROS generation
- Recommended for monitoring hydrogen peroxide production inside living cells under various physiological and pathological conditions

Available variants and fusions

HyPer comprises fluorescent part which codon usage is optimized for high expression in mammalian cells [Haas et al., 1996] and OxyR part from E. coli with autogenic codon usage. Effective expression of HyPer occures in both bacteria and mammalian cells.

HyPer-Cyto variant is localized in cell cytosol.

HyPer-dMito fusion: Mitochondrial targeting sequence (MTS) was derived from subunit VIII precursor of human cytochrome C oxidase [Rizzuto et al., 1989; Rizzuto et al., 1995]. Two MTS were fused to HyPer N-terminus. When expressed in mammalian cells, this variant is localized in mitochondria.

HyPer-AS variant: HyPer-AS codon usage is optimized for high expression in Arabidopsis and Saccharomyces. This variant is available in Gateway® entry clone.