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Kindling red fluorescent protein KFP-Red
- Reversible or irreversible photoactivation
- Activated by green light that does not damage cells and tissues
- Quenching by blue light
- Recommended for tracking cells and cellular organelle movements
Available variants and fusions
| Variant | Description | Related vector | Cat.# | Click for image |
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Humanized KFP-Red
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KFP-Red codon usage is optimized for high expression in mammalian cells [Haas et al., 1996], but it can be successfully expressed in many other heterological systems.
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pKindling-Red-N
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FP301
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pKindling-Red-B
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FP302
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KFP-Red-mito fusion
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A mitochondrial targeting sequence (MTS) is fused to the KFP-Red N-terminus. MTS was derived from the subunit VIII of human cytochrome C oxidase [Rizzuto et al., 1989; Rizzuto et al., 1995]. When expressed in mammalian cells, this variant provides red fluorescent labeling of mitochondria (after photoactivation).
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pKindling-Red-mito
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FP401
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References:
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Haas J, Park EC, Seed B.
Codon usage limitation in the expression of HIV-1 envelope glycoprotein.
Curr Biol. 1996; 6 (3):315-24. / pmid: 8805248
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Rizzuto R, Brini M, Pizzo P, Murgia M, Pozzan T.
Chimeric green fluorescent protein as a tool for visualizing subcellular organelles in living cells.
Curr Biol. 1995; 5 (6):635-42. / pmid: 7552174
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Rizzuto R, Nakase H, Darras B, Francke U, Fabrizi GM, Mengel T, Walsh F, Kadenbach B, DiMauro S, Schon EA.
A gene specifying subunit VIII of human cytochrome c oxidase is localized to chromosome 11 and is expressed in both muscle and non-muscle tissues.
J Biol Chem. 1989; 264 (18):10595-600. / pmid: 2543673
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