Cyan-to-green photoswitchable fluorescent protein PS-CFP2

- Irreversible photoconversion from a cyan to a green fluorescent form
- High contrast of photoconversion
- High pH stability allowing labeling of acidic organelles
- Recommended for tracking cell, organelle, and protein movement, and for determination of protein half-life

Available variants and fusions

PS-CFP was obtained from Aequorea coerulescens colorless GFP-like protein [Gurskaya et al., 2003]. As compared with PS-CFP, PS-CFP2 comprises S108T, M153V, and T154A substitutions, which lead to a much faster maturation and a brighter fluorescence both before and after photoswitching. PS-CFP2 codon usage is optimized for high expression in mammalian cells [Haas et al., 1996], but it can be successfully expressed in many other heterological systems.