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Werner ME, Hwang P, Huisman F, Taborek P, Yu CC, Mitchell BJ.
Actin and microtubules drive differential aspects of planar cell polarity in multiciliated cells.
J Cell Biol. 2011 Oct 3;195(1):19-26
pmid: 21949415
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Dower K, Rubins KH, Hensley LE, Connor JH.
Development of Vaccinia reporter viruses for rapid, high content analysis of viral function at all stages of gene expression.
Antiviral Res. 2011 Jul;91(1):72-80
pmid: 21569797
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Constructs used: coding sequence of TagBFP (pTagBFP-N vector, Evrogen) was inserted into vaccinia virus.
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Expression system: mammalian cell lines transduced with vaccinia virus.
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Detection system: Tecan Infinite M1000 fluorescent plate reader (TagBFP excitation at 415nm, emission colleted at 457 nm).
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Walther KA, Papke B, Sinn MB, Michel K, Kinkhabwala A.
Precise measurement of protein interacting fractions with fluorescence lifetime imaging microscopy.
Mol Biosyst. 2011 Feb;7(2):322-36
pmid: 21221430
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Constructs used: pTagBFP-N vector, Evrogen.
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Expression system: Madin-Darby canine kidney (MDCK) cell line transiently transfected with plasmid using Effectene transfection reagent; purified recombinant TagBFP.
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Detection system: fluorecence lifetime imaging in vitro and in vivo with Olympus Fluoview FV 1000 confocal microscope (excitation at 405 nm, emission detected with 430 LP filter set).
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DNA nicks promote efficient and safe targeted gene correction.
DNA nicks promote efficient and safe targeted gene correction.
PLoS One. 2011;6(9):e23981
pmid: 21912657
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Constructs used: mammalian expression vector containing EF1α promoter, T2A translational linker and TagBFP coding sequence.
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Expression system: HEK 293T cells.
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Detection system: FACS LSR II flow cytometer, TagBFP excitation with 405 nm laser.
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Bedoya L, Martínez F, Rubio L, Daròs JA.
Simultaneous equimolar expression of multiple proteins in plants from a disarmed potyvirus vector.
J Biotechnol. 2010 Oct 15;150(2):268-75
pmid: 20728479
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Constructs used: plant expression vector based on the potyvirus Tobacco etch virus (TEV) containing TagBFP coding sequence (from pTagBFP-N vector, Evrogen).
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Expression system: wild-type tobacco plants (Nicotiana tabacum and Nicotiana benthamiana) transduced by agroinoculation with Agrobacteriam tumefaciens.
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Detection system: fluorescence stereomiscroscope Leica MZ 16F and confocal microscope Leica TCS-SP2-AOBS (excitation of TagBFP with 405 nm laser, emission detected at 420-470nm).
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Niino Y, Hotta K, Oka K.
Blue fluorescent cGMP sensor for multiparameter fluorescence imaging.
PLoS One. 2010 Feb 11;5(2):e9164
pmid: 20161796
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Constructs used: constructed cGMP biosensor using the cGMP binding domain from a phosphodiesterase, an improved dark YFP (as a quenching acceptor) and TagBFP (as a blue fluorescent donor); TagBFP coding sequence was derived from pTagBFP-N vector, Evrogen.
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Expression system: mammalian cell lines transiently transfected with plasmid carrying the cGMP biosensor.
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Detection system: Olympus FluoView FV 1000 confocal laser scanning miscroscope; cGMP biosensor was observed in living cells by excitation with 405 nm laser and detection of emission at 440-480 nm.
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Subach OM, Gundorov IS, Yoshimura M, Subach FV, Zhang J, Gruenwald D, Souslova EA, Chudakov DM, Verkhusha VV.
Conversion of Red Fluorescent Protein into a Bright Blue Probe.
Chem Biol. 2008 Oct 20;15(10):1116-24
pmid: 18940671
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