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    pCase12-Cyto

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pCase12-Cyto vector

cat.# FP991

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

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vector information:
ProductCat.#SizePrice
pCase12-CytoFP99120 μg€ 600.00
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The product is not available in certain countries. Please contact Evrogen or your local distributor before ordering.

Vector typemammalian expression vector
ReporterCase12
Reporter codon usagemammalian
Promoter for Case12PCMV IE
Host cellsmammalian
Selectionprokaryotic - kanamycin
eukaryotic - neomycin (G418)
Replicationprokaryotic - pUC ori
eukaryotic - SV40 ori
Use Case12 expression in mammalian cells under the control of CMV promoter; source of Case12 coding sequence

Location of features

PCMV IE: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
Case12
Kozak consensus translation initiation site: 672-682
Start codon (ATG): 679-681
Stop codon: 1924-1926
Last amino acid in Case12: 1921-1923
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 1922-1927, 2080-2085 & 2109-2114
mRNA 3' ends: 2118 & 2130
f1 single-strand DNA origin: 2177-2632
Eukaryotic promoter for expression of Kanr gene
-35 region: 2694-2699
-10 region: 2717-2722
Transcription start point: 2729
SV40 origin of replication: 2973-3108
SV40 early promoter
Enhancer (72-bp tandem repeats): 2806-2877 & 2878-2949
21-bp repeats: 2953-2973, 2974-2994 & 2996-3016
Early promoter element: 3029-3035
Major transcription start points: 3025, 3063, 3069 & 3074
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 3157-3159
Stop codon: 3949-3951
G->A mutation to remove Pst I site: 3339
C->A (Arg to Ser) mutation to remove BssH II site: 3685
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 4187-4192 & 4200-4205
pUC plasmid replication origin: 4536-5179


Notice to Purchaser:

The Case12-related materials (also referred to as "Products") are intended for research use only.

Some elements of these materials may be also covered by third party patents issued and applicable in certain countries. No license under these patents is conveyed expressly or by implication to the recipient of the materials. Users of these materials may be required to obtain a patent license depending upon the particular application and country in which the materials are received or used.

The CMV promoter is covered under U.S. Patents 5,168,062 and 5,385,839, and its use is permitted for research purposes only. Any other use of the CMV promoter requires a license from the University of Iowa Research Foundation, 214 Technology Innovation Center, Iowa City, IA 52242.

MATERIAL SAFETY DATA SHEET INFORMATION

To the best of our knowledge, these products do not require a Material Safety Data Sheet. However, all the properties of these products (and, if applicable, each of their components) have not been thoroughly investigated. Therefore, we recommend that you use gloves and eye protection, and wear a laboratory coat when working with these products.

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