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RESEARCH PRODUCTS/Photoactivatable fluorescent proteins/PS-CFP2

pPS-CFP2-C

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pPS-CFP2-C vector

cat.# FP801

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

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vector information:

Product	Cat.#	Size	Price
pPS-CFP2-C	FP801	20 μg	€ 400.00
The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information.

Vector type	mammalian expression vector
Reporter	PS-CFP2
Reporter codon usage	mammalian
Promoter for PS-CFP2	P_{CMV IE}
Host cells	mammalian
Selection	prokaryotic - kanamycin eukaryotic - neomycin (G418)
Replication	prokaryotic - pUC ori eukaryotic - SV40 ori
Use	PS-CFP2 expression in mammalian cells; generation of fusions to the PS-CFP2 C-terminus

Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
PS-CFP2
Kozak consensus translation initiation site: 606-616
Start codon (ATG): 613-615
Stop codon: 1405-1407
Last amino acid in PS-CFP2: 1324-1326
MCS: 1327-1412
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 1547-1552 & 1576-1581
mRNA 3' ends: 1585 & 1597
f1 single-strand DNA origin: 1644-2099
Eukaryotic promoter for expression of Kan^r gene
-35 region: 2161-2166
-10 region: 2184-2189
Transcription start point: 2196
SV40 origin of replication: 2440-2575
SV40 early promoter
Enhancer (72-bp tandem repeats): 2273-2344 & 2345-2416
21-bp repeats: 2420-2440, 2441-2461 & 2463-2483
Early promoter element: 2496-2502
Major transcription start points: 2492, 2530, 2536 & 2541
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 2624-2626
Stop codon: 3416-3418
G->A mutation to remove Pst I site: 2806
C->A (Arg to Ser) mutation to remove BssH II site: 3152
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 3654-3659 & 3667-3672
pUC plasmid replication origin: 4003-4646

Notice to Purchaser:

Photoactivatable FP-related products are intended to be used by academic (non-commercial) entities and for research purposes only. Any use of the proprietary nucleic acid or protein other than for research use or by a commercial entity is strictly prohibited. Transfer of this product by purchaser to any other party is specifically prohibited.

The CMV promoter is covered under U.S. Patents 5,168,062 and 5,385,839, and its use is permitted for research purposes only. Any other use of the CMV promoter requires a license from the University of Iowa Research Foundation, 214 Technology Innovation Center, Iowa City, IA 52242.

MATERIAL SAFETY DATA SHEET INFORMATION

To the best of our knowledge, these products do not require a Material Safety Data Sheet. However, all the properties of these products (and, if applicable, each of their components) have not been thoroughly investigated. Therefore, we recommend that you use gloves and eye protection, and wear a laboratory coat when working with these products.


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