pTagRFP-mito vector

cat.# FP147

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

Download vector information: Data sheet (pdf) Sequence (txt) Restriction map (pdf) Restriction table (pdf)

Product Cat.# Size Price pTagRFP-mito FP147 20 μg € 400 The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information. Vector type mammalian expression vector Reporter TagRFP Reporter codon usage mammalian Promoter for TagRFP PCMV IE Host cells mammalian Selection prokaryotic – kanamycin eukaryotic – neomycin (G418) Replication prokaryotic – pUC ori eukaryotic – SV40 ori Use red (orange) fluorescent labeling of mitochondria

Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
TagRFP-mito fusion
Start codon (ATG): 597-599
Mitochondrial targeting sequence (MTS): 597-683
Start of TagRFP coding sequence (ATG): 705-707
Stop codon: 1416-1418
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 1571-1576 & 1600-1605
mRNA 3' ends: 1609 & 1621
f1 single-strand DNA origin: 1668-2123
Bacterial promoter for expression of Kan^r gene
-35 region: 2185-2190
-10 region: 2208-2213
Transcription start point: 2220
SV40 origin of replication: 2464-2599
SV40 early promoter
Enhancer (72-bp tandem repeats): 2297-2368 & 2369-2440
21-bp repeats: 2444-2464, 2465-2485 & 2487-2507
Early promoter element: 2520-2526
Major transcription start points: 2516, 2554, 2560 & 2565
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 2648-2650
Stop codon: 3440-3442
G->A mutation to remove Pst I site: 2830
C->A (Arg to Ser) mutation to remove BssH II site: 3176
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 3678-3683 & 3691-3696
pUC plasmid replication origin: 4027-4670