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Calcium ion sensor Case12
- High dynamic range calcium indicator
- Direct expression in cells
- High selectivity and sensitivity
- High brightness of fluorescent response
- Fast maturation at 37°C
- Relatively high pH stability
- Recommended for monitoring change of calcium concentration inside living cells
Main properties
Case12 normalized excitation (blue) and emission (green) spectra without Ca2+ (thin line) and in the presence of 1 mM of Ca2+ (thick line). | | CHARACTERISTIC | |
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| | Emission maximum, nm | 516 | | Excitation maximum, nm | 491 | | Fluorescence color | green | | Polypeptide length, aa | 415 | | Molecular weight, kDa | 46.4 | | Specificity | Ca2+ | | Kd for Ca2+ | 1 μM | | pKa | 7.2 | | Structure | monomer | | Aggregation | no | | Maturation rate at 37°C | fast |
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 | Ca2+ titration curves.The apparent Kd for calcium ions binding was found to be 1 μM, which lies within the physiological range of calcium ions concentrations. Image from Souslova et al., 2007. |
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References:
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Souslova EA, Belousov VV, Lock J, Stromblad S, Kasparov S, Bolshakov AP, Pinelis VG, Labas YA, Lukyanov S, Mayr LM, Chudakov DM.
Single fluorescent protein-based Ca2+ sensors with increased dynamic range.
BMC Biotechnol. 2007; 7 (1):37. / pmid: 17603870
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