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Calcium ion sensor Case12
- High dynamic range detection of intracellular Ca2+ level changes
- High selectivity and sensitivity, relatively high pH stability
- Fast maturation, high brightness of fluorescent response
- Direct expression in cells, easy targeting to various subcellular compartments
- No exogenous chemical compounds required
- Recommended for monitoring changes in Ca2+ concentration inside living cells
Available variants and fusions
| Variant | Description | Related vector | Cat.# | Click for image |
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Case12-cyto
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Case12 codon usage is optimized for high expression in mammalian cells [Haas et al., 1996], but it can be successfully expressed in other heterological systems.
When expressed in mammalian cells, this variant is evenly distributed throughout the cytosol and the nucleus.
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pCase12-cyto*
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FP991
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Gateway® Case12-cyto entry clone*
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FP994
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Case12-mito
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Mitochondrial targeting sequence derived from subunit VIII precursor of human cytochrome C oxidase [Rizzuto et al., 1989; Rizzuto et al., 1995] is fused to Case12 N-terminus. When expressed in mammalian cells, this variant is localized in mitochondria.
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pCase12-mito*
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FP992
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Gateway® Case12-mito entry clone*
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FP995
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Case12-mem
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Membrane localization signal of neuromodulin is fused to Case12 N-terminus. The signal provides for posttranslational palmitoylation that targets Case12 to cellular membranes [Skene and Virag, 1989].
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pCase12-mem*
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FP993
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References:
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Haas J, Park EC, Seed B.
Codon usage limitation in the expression of HIV-1 envelope glycoprotein.
Curr Biol. 1996; 6 (3):315-24. / pmid: 8805248
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Rizzuto R, Brini M, Pizzo P, Murgia M, Pozzan T.
Chimeric green fluorescent protein as a tool for visualizing subcellular organelles in living cells.
Curr Biol. 1995; 5 (6):635-42. / pmid: 7552174
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Rizzuto R, Nakase H, Darras B, Francke U, Fabrizi GM, Mengel T, Walsh F, Kadenbach B, DiMauro S, Schon EA.
A gene specifying subunit VIII of human cytochrome c oxidase is localized to chromosome 11 and is expressed in both muscle and non-muscle tissues.
J Biol Chem. 1989; 264 (18):10595-600. / pmid: 2543673
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Skene JH, Virag I.
Posttranslational membrane attachment and dynamic fatty acylation of a neuronal growth cone protein, GAP-43.
J Cell Biol. 1989; 108 (2):613-24. / pmid: 2918027
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