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RNA-i test vector, p2FP-RNAi

cat.# FP981

- Fast test of functional efficiency of synthetic siRNA
- Suitable internal control in the gene silencing experiments

p2FP-RNAi is a mammalian expression vector designed for RNAi studies. This dual-color vector encodes two fluorescent reporters: (1) red fluorescent protein JRed, that functions as a positive transfection marker; and (2) green fluorescent protein TurboGFP, that stands as a indicator of siRNA efficiency. Both proteins are under the control of immediate early promoter of cytomegalovirus (P_{CMV IE}). Multiple cloning site (MCS) of the vector allows to clone a gene or a gene fragment of interest into the TurboGFP 3' untranslated region (3'UTR).

When transfected into eukaryotic cells in the absence of a functional siRNA, the vector will express both JRed and TurboGFP proteins. In this case the brightness of green fluorescence significantly prevails.

In the presence of siRNA(s) directed against the cloned DNA fragment TurboGFP expression is knocking down. JRed expression at that remains unmodified or (in some experimental systems) increases due to translational competition. Thus, p2FP-RNAi vector makes it possible to trace RNA interference in the transfected cells by turning off/on green fluorescence against a background of red fluorescence.

vector use

vector map and description

Product	Cat.#	Size	Price
p2FP-RNAi vector	FP981	20 μg	€ 400
Price does not include delivery. Please contact your local distributor for exact prices and delivery information.

Notice to Purchaser:

Evrogen FP-related products are intended for research use only and covered by Evrogen Patents and/or Patent applications pending. By use of these products, you accept the terms and conditions of the applicable Limited Use Label License.

The CMV promoter is covered under U.S. Patents 5,168,062 and 5,385,839, and its use is permitted for research purposes only. Any other use of the CMV promoter requires a license from the University of Iowa Research Foundation, 214 Technology Innovation Center, Iowa City, IA 52242.

MATERIAL SAFETY DATA SHEET INFORMATION

To the best of our knowledge, these products do not require a Material Safety Data Sheet. However, all the properties of these products (and, if applicable, each of their components) have not been thoroughly investigated. Therefore, we recommend that you use gloves and eye protection, and wear a laboratory coat when working with these products.


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