RESEARCH PRODUCTS/Basic fluorescent proteins/TagFPs:


    TagCFP

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Cyan fluorescent protein TagCFP

- Bright cyan fluorescence
- Monomeric protein with successful performance in fusions
- High pH stability and photostability
- Recommended for protein labeling

Performance and use

TagCFP can be easily expressed and detected in a wide range of organisms. Mammalian cells transiently transfected with TagCFP expression vectors give bright fluorescent signals within 10-12 hrs after transfection. No cell toxic effects and visible protein aggregation are observed.

TagCFP performance in fusions has been demonstrated in human cytoplasmic β-actin, α-tubulin, and mitochondria models.

TagCFP use for protein labeling.

Confocal microscopy of transiently transfected mammalian cells expressing TagCFP-tagged fusions: (A) α-tubulin; (B) β-actin.

See also 3D vizualization of TagCFP-α-tubulin fusion

TagCFP suitability to generate stably transfected cells has been proven by Marinpharm company. Cell line expressing TagCFP fusion with mitochondrial targeting sequence (MTS) is commercially available.

TagCFP use for labeling of cells and cellular orgenelles.

(A) Confocal microscopy of transiently transfected mammalian cells expressing TagCFP; (B) Mitochondria-targeted TagCFP in stably transfected U-205 cell line.
Image was kindly provided by Dr. Christian Petzelt (Marinpharm).

TagCFP can be used in multicolor labeling applications with green, yellow, red, and far-red fluorescent dyes.

TagCFP use in multicolor labeling of mammalian cells.

(A) TagCFP-tagged β-actin (cyan), TagYFP-tagged α-tubulin (green), TagFP635-H2B fusion (red), and Golgi-targeted TagRFP (orange); (B) mitochondria-targeted TagCFP (cyan), peroxisome-targeted PhiYFP-m (yellow), and membrane-targeted KillerRed (red); (C) TagCFP-tagged α-tubulin (cyan), TagFP635-clathrin fusion (red), mitochondria-targeted TagYFP (green). Images (A, C) were kindly provided by Michael W. Davidson (Florida State University).

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