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Cyan fluorescent protein TagCFP
- Bright cyan fluorescence
- Monomeric protein with successful performance in fusions
- Fast maturation, high pH-stability and photostability
- Proven suitability to generate stably transfected cell lines
- Recommended for protein labeling
Available variants and fusions
| Variant | Description | Related vector | Cat.# | Click for image |
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Humanized TagCFP
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TagCFP codon usage is optimized for high expression in mammalian cells [Haas et al., 1996], but it can be successfully expressed in many other heterological systems.
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pTagCFP-C
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FP111
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pTagCFP-N
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FP112
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TagCFP-actin fusion
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Human β-actin is fused to the TagCFP C-terminus. When expressed in mammalian cells, this fusion provides cyan fluorescent labeling of β-actin in living cells.
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pTagCFP-actin
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FP114
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TagCFP-tubulin fusion
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Human α-tubulin is fused to the TagCFP C-terminus. When expressed in mammalian cells, this fusion provides cyan fluorescent labeling of α-tubulin in living cells.
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pTagCFP-tubulin
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FP115
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TagCFP-mito fusion
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A mitochondrial targeting sequence (MTS) is fused to the TagCFP N-terminus. MTS was derived from the subunit VIII of human cytochrome C oxidase [Rizzuto et al., 1989; Rizzuto et al., 1995]. When expressed in mammalian cells, this variant provides cyan fluorescent labeling of mitochondria.
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pTagCFP-mito
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FP117
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TagCFP-keratin fusion
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Human cytokeratin-18 is fused to the TagCFP N-terminus. When expressed in mammalian cells, this fusion provides cyan fluorescent labeling of cytokeratin-18 in living cells.
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pTagCFP-keratin
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FP119
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References:
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Haas J, Park EC, Seed B.
Codon usage limitation in the expression of HIV-1 envelope glycoprotein.
Curr Biol. 1996; 6 (3):315-24. / pmid: 8805248
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Rizzuto R, Brini M, Pizzo P, Murgia M, Pozzan T.
Chimeric green fluorescent protein as a tool for visualizing subcellular organelles in living cells.
Curr Biol. 1995; 5 (6):635-42. / pmid: 7552174
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Rizzuto R, Nakase H, Darras B, Francke U, Fabrizi GM, Mengel T, Walsh F, Kadenbach B, DiMauro S, Schon EA.
A gene specifying subunit VIII of human cytochrome c oxidase is localized to chromosome 11 and is expressed in both muscle and non-muscle tissues.
J Biol Chem. 1989; 264 (18):10595-600. / pmid: 2543673
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