Yellow fluorescent protein TagYFP

- Bright yellow fluorescence
- Monomeric protein with successful performance in fusions
- Fast maturation, high pH-stability and photostability
- Proven suitability to generate stably transfected cell lines
- Recommended for protein labeling

Performance and use

TagYFP can be easily expressed and detected in a wide range of organisms. Mammalian cells transiently transfected with TagYFP expression vectors give bright fluorescent signals in 10-12 hrs after transfection. No cytotoxic effects or visible protein aggregation are observed.

		TagYFP use for labeling of cells and cellular orgenelles. Confocal microscopy of transiently transfected mammalian cells expressing TagYFP.

TagYFP performance in fusions has been demonstrated in human cytoplasmic β-actin and α-tubulin models. An expected pattern of fluorescence has been obtained in each case.

		TagYFP use for protein labeling. Confocal microscopy of transiently transfected mammalian cells expressing TagYFP fusions.

TagYFP suitability to generate stably transfected cells has been proven by Marinpharm company. Cell lines expressing TagYFP are commercially available.

		Stably transfected cell lines expressing TagYFP fusions. (A) MDCK cells expressing TagYFP-tubulin fusion; (B) U-205 cells expressing TagYFP-actin fusion. Images were kindly provided by Dr. Christian Petzelt (Marinpharm).

TagYFP can be used in multicolor labeling applications with blue, cyan, red, and far-red fluorescent dyes.

		Multicolor labeling using TagFPs. (A) TagCFP-tagged β-actin (cyan), TagYFP-tagged α-tubulin (yellow), TagFP635-H2B fusion (red), and Golgi-targeted TagRFP (violet); (B) TagCFP-tagged α-tubulin (cyan), TagFP635-clathrin fusion (red), mitochondria-targeted TagYFP (yellow). Images were kindly provided by Michael W. Davidson (Florida State University).