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Yellow fluorescent protein TagYFP
- Bright yellow fluorescence
- Monomeric protein with successful performance in fusions
- High pH stability and photostability
- Recommended for protein labeling
Available variants and fusionsTagYFP codon usage is optimized for high expression in mammalian cells [Haas et al., 1996], but it can be successfully expressed in many other heterological systems. TagYFP-mito fusion: A mitochondrial targeting sequence (MTS) is linked to the TagYFP N-terminus. MTS was derived from the subunit VIII of human cytochrome C oxidase [Rizzuto et al., 1989; Rizzuto et al., 1995]. When expressed in mammalian cells, this variant provides yellow fluorescent labeling of mitochondria. TagYFP-actin fusion: Human β-actin is linked to the TagYFP C-terminus. When expressed in mammalian cells, this fusion provides yellow fluorescent labeling of actin filaments. TagYFP-tubulin fusion: Human α-tubulin is linked to the TagYFP C-terminus. When expressed in mammalian cells, this fusion provides yellow fluorescent labeling of tubulin filaments.
References:
- Haas J, Park EC, Seed B. Codon usage limitation in the expression of HIV-1 envelope glycoprotein. Curr Biol. 1996; 6 (3):315-24. / pmid: 8805248
- Rizzuto R, Brini M, Pizzo P, Murgia M, Pozzan T. Chimeric green fluorescent protein as a tool for visualizing subcellular organelles in living cells. Curr Biol. 1995; 5 (6):635-42. / pmid: 7552174
- Rizzuto R, Nakase H, Darras B, Francke U, Fabrizi GM, Mengel T, Walsh F, Kadenbach B, DiMauro S, Schon EA. A gene specifying subunit VIII of human cytochrome c oxidase is localized to chromosome 11 and is expressed in both muscle and non-muscle tissues. J Biol Chem. 1989; 264 (18):10595-600. / pmid: 2543673
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