Anti-Dendra2 antibody

cat.# AB821, AB822

Rabbit polyclonal antibody against Dendra2

PRODUCT	CAT.#	SIZE	PRICE	DATA SHEET (PDF)
The prices do not include delivery. The prices vary in different countries. Please contact your local distributor for exact prices and delivery information.
Anti-Dendra2 antibody	AB821	100 μg	€ 150
	AB822	200 μg	€ 200

Specificity
The antibody has been selected to recognize both denatured and native Dendra2. The antibody shows little or no cross-reactivity with TagCFP, TagGFP, TagGFP2, TagYFP, TagRFP, TurboGFP, TurboYFP, TurboRFP, TurboFP602, JRed, and KillerRed.

Western blot detection of fluorescent proteins using Anti-Dendra2 antibody. 1 — TagCFP; 2 — TagGFP; 3 — TagYFP; 4 — TagRFP; 5 — TurboFP602; 6 — TurboGFP; 7 — TurboYFP; 8 — TurboRFP; 9 — PS-CFP2; 10 — Dendra2; 11 — KillerRed; 12 — EGFP. Recombinant proteins were purified from transformed E. coli. 25 ng of each protein were separated by SDS PAGE (14% acrylamide). The samples were boiled before loading. Antibody was used at a 1/10000 dilution. Secondary antibody: Goat anti-Rabbit HRP-conjugated IgG.

Immunogen
Full-length recombinant denatured Dendra2.

Antibody preparation
Full-length recombinant Dendra2 was purified from transformed E. coli using organic extraction and hydrophobic chromatography. Antibody was produced in rabbits immunized with the recombinant denatured Dendra2 and purified by Dendra2 affinity chromatography.

Formulation
Lyophilized from the buffer containing 0.01M Na₂PO₄, 0.1M NaCl, 0.25 mg/ml gelatin, and 1% trehalose; pH 7.4.

Reconstitution
Reconstitute with sterile water or 50% glycerol.

Storage
Lyophilized samples are stable for twelve months from date of receipt when stored at -20°C. The presence of silica gel drier is advisable.
Reconstituted with sterile water, antibody can be stored at +2 - +8°C for three months without detectable loss of activity.
Reconstituted with 50% glycerol, antibody can be stored at -20°C in a manual defrost freezer for six months without detectable loss of activity. Aliquot antibody upon reconstitution. Avoid repeated freeze / thaw cycles.

Use

• Western blot
• Immunoblotting
• ICC
• ELISA