Anti-PhiYFP antibody

cat.# AB601, AB602

Rabbit polyclonal antibody against non-denatured PhiYFP, PhiYFP-m, and TurboYFP

PRODUCT	CAT.#	SIZE	PRICE	DATA SHEET
Anti-PhiYFP antibody	AB601	100 μg	€ 150.00	AB601
	AB602	200 μg	€ 200.00	AB602
The prices do not include delivery. The prices vary in different countries. Please contact your local distributor for exact prices and delivery information.

Specificity
The antibody has been selected to recognize non-denatured TurboYFP, PhiYFP, and PhiYFP-m. Heat or chemically denatured proteins lack antigen determinants. The antibody shows little or no cross-reactivity with other fluorescent proteins like EGFP, TurboGFP, KFP-Red, and DsRed2.

Immunogen
Full-length recombinant non-denatured PhiYFP comprising 6XHis tag.

Antibody preparation
Full-length recombinant PhiYFP comprising 6XHis tag was purified from transformed E. coli using metal-ion affinity chromatography. Antibodies were produced in rabbits immunized with the recombinant non-denaturated PhiYFP. Specific IgG were purified by PhiYFP affinity chromatography.

Formulation
Lyophilized from the buffer containing 0.1% mannitol, 0.1% dextran, 0.1M NaCl, 0.01M Na₂PO₄, and 0.01M NaBO₄; pH 7.4.

Reconstitution
Reconstitute with sterile water or 50% glycerol.

Storage
Lyophilized samples are stable for twelve months from date of receipt when stored at -20°C. The presence of silica gel drier is advisable.
Reconstituted with sterile water, antibody can be stored at +2 - +8°C for three months without detectable loss of activity.
Reconstituted with 50% glycerol, antibody can be stored at -20°C in a manual defrost freezer for six months without detectable loss of activity. Aliquot antibody upon reconstitution. Avoid repeated freeze / thaw cycles.

Use

• Immunoblotting
• Immunohistochemistry
• ELISA
• In cell Western
• Immunoprecipitation