Anti-PhiYFP(d) antibody

cat.# AB603, AB604

Rabbit polyclonal antibody against denatured PhiYFP, PhiYFP-m, and TurboYFP

PRODUCT	CAT.#	SIZE	PRICE	DATA SHEET
Anti-PhiYFP(d) antibody	AB603	100 μg	€ 150.00	AB603
	AB604	200 μg	€ 200.00	AB604
The prices do not include delivery. The prices vary in different countries. Please contact your local distributor for exact prices and delivery information.

Specificity
The antibody has been selected to recognize denatured TurboYFP, PhiYFP, and PhiYFP-m. It can also be used for recognizing non-denatured Phi-Yellow proteins, but with lesser activity than Anti-PhiYFP (Cat.# AB601-AB602) antibody. The antibody shows little or no cross-reactivity with TagCFP, TagGFP, TagYFP, TagRFP, TurboGFP, TurboRFP, JRed, Dendra2 and KillerRed. The antibody shows cross-reactivity with TagRFP, TurboFP602, and TurboFP635.

Western blot detection of fluorescent proteins using Anti-PhiYFP(d) antibody. 1 — TagCFP; 2 — TagGFP; 3 — TagYFP; 4 — TagRFP; 5 — TurboFP602; 6 — TurboGFP; 7 — TurboYFP; 8 — TurboRFP; 9 — PS-CFP2; 10 — Dendra2; 11 — KillerRed; 12 — EGFP. Recombinant proteins were purified from transformed E. coli. 25 ng of each protein were separated by SDS PAGE (14% acrylamide). The samples were boiled before loading. Antibody was used at a 1/10000 dilution. Secondary antibody: Goat anti-Rabbit HRP-conjugated IgG.

Immunogen
Full-length recombinant denatured PhiYFP comprising 6XHis tag.

Antibody preparation
Full-length recombinant PhiYFP comprising 6XHis tag was purified from transformed E. coli using metal-ion affinity chromatography. Antibodies were produced in rabbits immunized with the recombinant denaturated PhiYFP. Specific IgG were purified by PhiYFP affinity chromatography.

Formulation
Lyophilized from the buffer containing 0.1% mannitol, 0.1% dextran, 0.1M NaCl, 0.01M Na₂PO₄, and 0.01M NaBO₄; pH 7.4.

Reconstitution
Reconstitute with sterile water or 50% glycerol.

Storage
Lyophilized samples are stable for twelve months from date of receipt when stored at -20°C. The presence of silica gel drier is advisable.
Reconstituted with sterile water, antibody can be stored at +2 - +8°C for three months without detectable loss of activity.
Reconstituted with 50% glycerol, antibody can be stored at -20°C in a manual defrost freezer for six months without detectable loss of activity. Aliquot antibody upon reconstitution. Avoid repeated freeze / thaw cycles.

Use

• Western blot
• Immunoblotting
• Immunohistochemistry
• ELISA
• In cell Western
• Immunoprecipitation