pCasper3-BG vector

cat.# FP970

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

Download vector information: Data sheet (pdf) Sequence (txt) Restriction map (pdf) Restriction table (pdf)

Product Cat.# Size Price pCasper3-BG FP970 20 μg € 600 The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information. Vector type mammalian expression vector Reporter Casper3-BG Reporter codon usage mammalian Promoter for Casper3-BG PCMV IE Host cells mammalian Selection prokaryotic – kanamycin eukaryotic – neomycin (G418) Replication prokaryotic – pUC ori eukaryotic – SV40 ori Use Expression of fluorescent caspase-3 apoptosis sensor Casper3-BG in mammalian cells under the control of CMV promoter; source of Casper3-BG coding sequence

Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
Casper3-BG
Kozak consensus translation initiation site: 672-682
Start codon (ATG): 679-681
TagBFP: 679-1374
Linker: 1375-1424
TagGFP2: 1425-2151
Stop codon: 2149-2151
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 2304-2309 & 2333-2338
mRNA 3' ends: 2342 & 2354
f1 single-strand DNA origin: 2401-2856
Bacterial promoter for expression of Kan^r gene
-35 region: 2918-2923
-10 region: 2941-2946
Transcription start point: 2953
SV40 origin of replication: 3197-3332
SV40 early promoter
Enhancer (72-bp tandem repeats): 3030-3101 & 3102-3173
21-bp repeats: 3177-3197, 3198-3218 & 3220-3240
Early promoter element: 3253-3259
Major transcription start points: 3249, 3287, 3293 & 3298
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 3381-3383
Stop codon: 4173-4175
G->A mutation to remove Pst I site: 3563
C->A (Arg to Ser) mutation to remove BssH II site: 3909
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 4411-4416 & 4424-4429
pUC plasmid replication origin: 4760-5403