RESEARCH PRODUCTS/Photoactivatable fluorescent proteins/Dendra2


    pDendra2-B

pDendra2-B vector

cat.# FP823

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

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vector information:
ProductCat.#SizePrice
pDendra2-BFP82320 μg€ 400.00
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Vector typebacterial expression vector
ReporterDendra2
Reporter codon usagemammalian
Promoter for Dendra2T5 promoter/lac operator
Host cellsprokaryotic
Selectionampicilin
ReplicationColE1 ori
Use Source of the mammalian Dendra2 coding sequence; Dendra2 expression in bacterial cells
5'-Region
Dendra2
BamH I
RBS ATG. AGA.GGA.TCG. GGA.TCC. ATG.AAC.ACC.CCG...
3'-Region
Stop
Hind III
...GTG.TAC.AAG. TGA. AAG.CTT

Vector description

pDendra2-B is a a prokaryotic expression vector encoding green-to-red fluorescent protein Dendra2 (see protein description). Reporter codon usage is optimized for high expression in mammalian cells (humanized) [Haas et al., 1996].

The vector is primarily intended as a source of Dendra2 coding sequence. Flanking restriction sites are convenient for Dendra2 gene excision and its further insertion into other expression vectors of choice. Alternatively, Dendra2 coding sequence can be amplified by PCR.

Note: The plasmid DNA was isolated from dam+-methylated E.coli. Therefore some restriction sites are blocked by methylation. If you wish to digest the vector using such sites you will need to transform the vector into a dam- host and make fresh DNA.

The vector can be also used for Dendra2 expression in prokaryotes under the control of T5 promoter/lac operator. The vector backbone contains ColE1 origin of replication and ampicillin resistance gene for propagation and selection in E. coli.


Location of features

T5 promoter/lac operator element: 7-87
T5 transcription start: 61
DendraV coding sequence: 133-822
Lambda t0 transcriptional termination region: 871-965
rrnB T1 transcriptional termination region: 1727-1825
ColE1 origin of replication: 2301
beta-lactamase coding sequence: 3919-3059


References:

  • Haas J, Park EC, Seed B. Codon usage limitation in the expression of HIV-1 envelope glycoprotein. Curr Biol. 1996; 6 (3):315-24. / pmid: 8805248

Notice to Purchaser:

Photoactivatable FP-related products are intended to be used by academic (non-commercial) entities and for research purposes only. Any use of the proprietary nucleic acid or protein other than for research use or by a commercial entity is strictly prohibited. Transfer of this product by purchaser to any other party is specifically prohibited.

MATERIAL SAFETY DATA SHEET INFORMATION

To the best of our knowledge, these products do not require a Material Safety Data Sheet. However, all the properties of these products (and, if applicable, each of their components) have not been thoroughly investigated. Therefore, we recommend that you use gloves and eye protection, and wear a laboratory coat when working with these products.

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