RESEARCH PRODUCTS/Photoactivatable fluorescent proteins/KFP-Red


    pKindling-Red-mito

SEARCH

pKindling-Red-mito vector

cat.# FP401

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

Download
vector information:
ProductCat.#SizePrice
pKindling-Red-mitoFP40120 μg€ 400.00
The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information.

Vector typemammalian expression vector
ReporterKFP-Red
Reporter codon usagemammalian
Promoter for KFP-RedPCMV IE
Host cellsmammalian
Selectionprokaryotic - kanamycin
eukaryotic - neomycin (G418)
Replicationprokaryotic - pUC ori
eukaryotic - SV40 ori
Use NO/red fluorescent labeling of mitochondria

Location of features

PCMV IE: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
KFP-Red-mito fusion
Start codon (ATG): 597-599
Mitochondrial targeting sequence (MTS): 597-683
Start of XGFP coding sequence (ATG): 705-707
Stop codon: 1401-1403
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 1556-1561 & 1585-1590
mRNA 3' ends: 1594 & 1606
f1 single-strand DNA origin: 1653-2108
Eukaryotic promoter for expression of Kanr gene
-35 region: 2170-2175
-10 region: 2193-2198
Transcription start point: 2205
SV40 origin of replication: 2449-2584
SV40 early promoter
Enhancer (72-bp tandem repeats): 2282-2353 & 2354-2425
21-bp repeats: 2429-2449, 2450-2470 & 2472-2492
Early promoter element: 2505-2511
Major transcription start points: 2501, 2539, 2545 & 2550
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 2633-2635
Stop codon: 3425-3427
G->A mutation to remove Pst I site: 2815
C->A (Arg to Ser) mutation to remove BssH II site: 3161
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 3663-3668 & 3676-3681
pUC plasmid replication origin: 4012-4655


Notice to Purchaser:

Photoactivatable FP-related products are intended to be used by academic (non-commercial) entities and for research purposes only. Any use of the proprietary nucleic acid or protein other than for research use or by a commercial entity is strictly prohibited. Transfer of this product by purchaser to any other party is specifically prohibited.

The CMV promoter is covered under U.S. Patents 5,168,062 and 5,385,839, and its use is permitted for research purposes only. Any other use of the CMV promoter requires a license from the University of Iowa Research Foundation, 214 Technology Innovation Center, Iowa City, IA 52242.

MATERIAL SAFETY DATA SHEET INFORMATION

To the best of our knowledge, these products do not require a Material Safety Data Sheet. However, all the properties of these products (and, if applicable, each of their components) have not been thoroughly investigated. Therefore, we recommend that you use gloves and eye protection, and wear a laboratory coat when working with these products.

Copyright 2002-2008 Evrogen. All rights reserved.
Evrogen JSC, 16/10 Miklukho-Maklaya str., Moscow, Russia, +7(495)4298020, e-mail:evrogen@evrogen.com