pPhi-Yellow-B vector

cat.# FP603

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

Download vector information: Data sheet (pdf) Sequence (txt) Restriction map (pdf) Restriction table (pdf)

Product Cat.# Size Price pPhi-Yellow-B FP603 20 μg € 400 The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information. Vector type bacterial expression vector Reporter PhiYFP Reporter codon usage mammalian Promoter for PhiYFP T5 promoter/lac operator Host cells prokaryotic Selection ampicillin Replication ColE1 ori Use Source of the PhiYFP coding sequence; PhiYFP expression in bacterial cells

5'-Region

				PhiYFP
			BamH I
RBS	ATG.	AGA.GGA.TCG.	GGA.	TCC.	AGC.GGC.GCC...

3'-Region

	Stop
		Hind III
...ACC.TAC.CTG.	TG	A.	AGC.TT

Vector description

pPhi-Yellow-B is a prokaryotic expression vector encoding yellow fluorescent protein PhiYFP (see reporter description). Reporter codon usage is optimized for high expression in mammalian cells (humanized) [Haas et al., 1996].

The vector is primarily intended as a source of PhiYFP coding sequence. Flanking restriction sites are convenient for excision of PhiYFP sequence and its further insertion into other expression vectors of choice. Alternatively, PhiYFP coding sequence can be amplified by PCR.

The vector can be also used for PhiYFP expression in prokaryotes under the control of T5 promoter/lac operator. The vector backbone contains ColE1 origin of replication and ampicillin resistance gene for propagation and selection in E. coli.

Location of features

T5 promoter/lac operator element: 7-87
T5 transcription start: 61
PhiYFP coding sequence: 130-831
Lambda t0 transcriptional termination region: 852-946
rrnB T1 transcriptional termination region: 1708-1806
ColE1 origin of replication: 2282
beta-lactamase coding sequence: 3900-3040