pTurboFP602-B vector

cat.# FP713

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

Download vector information: Data sheet (pdf) Sequence (txt) Restriction map (pdf) Restriction table (pdf)

Product Cat.# Size Price pTurboFP602-B FP713 20 μg € 400 The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information. Vector type bacterial expression vector Reporter TurboFP602 Reporter codon usage mammalian Promoter for TurboFP602 T5 promoter/lac operator Host cells prokaryotic Selection ampicillin Replication ColE1 ori Use Source of the TurboFP602 coding sequence; TurboFP602 expression in bacterial cells

5'-Region

				TurboFP602
			BamH I
RBS	ATG.	AGA.GGA.TCG.	GGA.TCC.	ATG.GTG.GGT.GAG...

3'-Region

	Stop
		Hind III
...GGG.CAC.AGC.	TG	A.	AG.CTT

Vector description

pTurboFP602-B is a prokaryotic expression vector encoding true-red fluorescent protein TurboFP602 (see reporter description). Reporter codon usage is optimized for high expression in mammalian cells (humanized) [Haas et al., 1996].

The vector is primarily intended as a source of TurboFP602 coding sequence. Flanking restriction sites are convenient for excision of TurboFP602 sequence and its further insertion into other expression vectors of choice. Alternatively, TurboFP602 coding sequence can be amplified by PCR.

The vector can be also used for TurboFP602 expression in prokaryotes under the control of T5 promoter/lac operator. The vector backbone contains ColE1 origin of replication and ampicillin resistance gene for propagation and selection in E. coli.

Location of features

T5 promoter/lac operator element: 7-87
T5 transcription start: 61
TurboFP602 coding sequence: 133-840
Lambda t0 transcriptional termination region: 861-955
rrnB T1 transcriptional termination region: 1717-1815
ColE1 origin of replication: 2291
beta-lactamase coding sequence: 3909-3049