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The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced. |
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Location of features
MCS: 12-89
TurboRFP-dest1
Kozak consensus translation initiation site: 90-100
Start codon (ATG): 97-99
Last amino acid in TurboGFP: 799-801
Stop codon: 937-939
MODC PEST sequence: 817-939
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 1094-1099 & 1123-1128
mRNA 3' ends: 1132 & 1144
f1 single-strand DNA origin: 1191-1646
Eukaryotic promoter for expression of Kanr gene
-35 region: 1708-1713
-10 region: 1731-1736
Transcription start point: 1743
SV40 origin of replication: 1987-2122
SV40 early promoter
Enhancer (72-bp tandem repeats): 1820-1891 & 1892-1963
21-bp repeats: 1967-1987, 1988-2008 & 2010-2030
Early promoter element: 2043-2049
Major transcription start points: 2039, 2077, 2083 & 2088
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 2171-2173
Stop codon: 2963-2965
G->A mutation to remove Pst I site: 2353
C->A (Arg to Ser) mutation to remove BssH II site: 2699
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 3201-3206 & 3214-3219
pUC plasmid replication origin: 3550-4193
Notice to Purchaser:
Evrogen Fluorescent Protein Products (the Products) are intended for research use only and covered by Evrogen Patents and/or Patent applications pending. By use of these products, you accept the terms and conditions of the applicable Limited Use Label License.
MATERIAL SAFETY DATA SHEET INFORMATION
To the best of our knowledge, these products do not require a Material Safety Data Sheet. However, all the properties of these products (and, if applicable, each of their components) have not been thoroughly investigated. Therefore, we recommend that you use gloves and eye protection, and wear a laboratory coat when working with these products.
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Copyright 2002-2008 Evrogen. All rights reserved. Evrogen JSC, 16/10 Miklukho-Maklaya str., Moscow, Russia, +7(495)4298020, e-mail:evrogen@evrogen.com |