pTurboRFP-PRL-dest1 vector

cat.# FP238

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

Download vector information: Data sheet (pdf) Sequence (txt) Restriction map (pdf) Restriction table (pdf)

Product Cat.# Size Price pTurboRFP-PRL-dest1 FP238 20 μg € 400 The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information. Vector type promoterless expression vector Reporter TurboRFP Reporter codon usage mammalian Promoter for TurboRFP NO Host cells mammalian, prokaryotic Selection prokaryotic – kanamycin eukaryotic – neomycin (G418) Replication prokaryotic – pUC ori eukaryotic – SV40 ori Use Monitoring of activity of different promoters and promoter/enhancer combinations

Location of features

MCS: 12-89
TurboRFP-dest1
Kozak consensus translation initiation site: 90-100
Start codon (ATG): 97-99
Last amino acid in TurboRFP: 799-801
Stop codon: 937-939
MODC PEST sequence: 817-939
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 1094-1099 & 1123-1128
mRNA 3' ends: 1132 & 1144
f1 single-strand DNA origin: 1191-1646
Bacterial promoter for expression of Kan^r gene
-35 region: 1708-1713
-10 region: 1731-1736
Transcription start point: 1743
SV40 origin of replication: 1987-2122
SV40 early promoter
Enhancer (72-bp tandem repeats): 1820-1891 & 1892-1963
21-bp repeats: 1967-1987, 1988-2008 & 2010-2030
Early promoter element: 2043-2049
Major transcription start points: 2039, 2077, 2083 & 2088
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 2171-2173
Stop codon: 2963-2965
G->A mutation to remove Pst I site: 2353
C->A (Arg to Ser) mutation to remove BssH II site: 2699
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 3201-3206 & 3214-3219
pUC plasmid replication origin: 3550-4193