pTurboYFP-PRL vector

cat.# FP615

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

Download vector information: Data sheet (pdf) Sequence (txt) Restriction map (pdf) Restriction table (pdf)

Product Cat.# Size Price pTurboYFP-PRL FP615 20 μg € 400.00 The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information. Vector type promoterless expression vector Reporter TurboYFP Reporter codon usage mammalian Promoter for TurboYFP NO Host cells mammalian, prokaryotic Selection prokaryotic - kanamycin eukaryotic - neomycin (G418) Replication prokaryotic - pUC ori eukaryotic - SV40 ori Use Monitoring of activity of different promoters and promoter/enhancer combinations

Location of features

MCS: 12-89
TurboYFP
Kozak consensus translation initiation site: 90-100
Start codon (ATG): 97-99
Stop codon: 826-828
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 982-987 & 1011-1016
mRNA 3' ends: 1020 & 1032
f1 single-strand DNA origin: 1079-1534
Eukaryotic promoter for expression of Kan^r gene
-35 region: 1596-1601
-10 region: 1619-1624
Transcription start point: 1631
SV40 origin of replication: 1875-2010
SV40 early promoter
Enhancer (72-bp tandem repeats): 1708-1779 & 1780-1851
21-bp repeats: 1855-1875, 1876-1896 & 1898-1918
Early promoter element: 1931-1937
Major transcription start points: 1927, 1965, 1971 & 1976
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 2059-2061
Stop codon: 2851-2853
G->A mutation to remove Pst I site: 2241
C->A (Arg to Ser) mutation to remove BssH II site: 2587
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 3089-3094 & 3102-3107
pUC plasmid replication origin: 3438-4081