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    pTurboYFP-dest1

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pTurboYFP-dest1 vector

cat.# FP619

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

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vector information:
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pTurboYFP-dest1FP61920 μg€ 400.00
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Vector typemammalian expression vector
ReporterTurboYFP
Reporter codon usagemammalian
Promoter for TurboYFPPCMV IE
Host cellsmammalian
Selectionprokaryotic - kanamycin
eukaryotic - neomycin (G418)
Replicationprokaryotic - pUC ori
eukaryotic - SV40 ori
Use TurboYFP expression in mammalian cells; generation of fusions to the TurboYFP-dest1 N-terminus

Location of features

PCMV IE: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
MCS: 591-671
TurboYFP
Kozak consensus translation initiation site: 672-682
Start codon (ATG): 679-681
Last amino acid in TurboYFP: 1405-1407
Stop codon: 1549-1551
MODC PEST sequence: 1426-1548
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 1706-1711 & 1735-1740
mRNA 3' ends: 1744 & 1756
f1 single-strand DNA origin: 1803-2258
Eukaryotic promoter for expression of Kanr gene
-35 region: 2320-2325
-10 region: 2343-2348
Transcription start point: 2355
SV40 origin of replication: 2599-2734
SV40 early promoter
Enhancer (72-bp tandem repeats): 2432-2503 & 2504-2575
21-bp repeats: 2579-2599, 2600-2620 & 2622-2642
Early promoter element: 2655-2661
Major transcription start points: 2651, 2689, 2695 & 2700
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 2783-2785
Stop codon: 3575-3577
G->A mutation to remove Pst I site: 2965
C->A (Arg to Ser) mutation to remove BssH II site: 3311
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 3813-3818 & 3826-3831
pUC plasmid replication origin: 4162-4805


Notice to Purchaser:

Evrogen Fluorescent Protein Products (the Products) are intended for research use only and covered by Evrogen Patents and/or Patent applications pending. By use of these products, you accept the terms and conditions of the applicable Limited Use Label License.

The CMV promoter is covered under U.S. Patents 5,168,062 and 5,385,839, and its use is permitted for research purposes only. Any other use of the CMV promoter requires a license from the University of Iowa Research Foundation, 214 Technology Innovation Center, Iowa City, IA 52242.

MATERIAL SAFETY DATA SHEET INFORMATION

To the best of our knowledge, these products do not require a Material Safety Data Sheet. However, all the properties of these products (and, if applicable, each of their components) have not been thoroughly investigated. Therefore, we recommend that you use gloves and eye protection, and wear a laboratory coat when working with these products.

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