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    pmKate2-keratin

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pmKate2-keratin vector

cat.# FP319

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

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vector information:
ProductCat.#SizePrice
pmKate2-keratinFP31920 μg€ 400
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Vector typemammalian expression vector
ReportermKate2
Reporter codon usagemammalian
Promoter for mKate2PCMV IE
Host cellsmammalian
Selectionprokaryotic – kanamycin
eukaryotic – neomycin (G418)
Replicationprokaryotic – pUC ori
eukaryotic – SV40 ori
Use far-red fluorescent labeling of cytokeratin-18

Location of features

PCMV IE: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
Keratin-mKate2 fusion: 600-2636
Keratin: 600-1889
Start codon (ATG): 600-602
Last amino acid in Keratin: 1887-1889
mKate2: 1941-2636
Stop codon: 2637-2639
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 2792-2797 & 2821-2826
mRNA 3' ends: 2830 & 2842
f1 single-strand DNA origin: 2889-3344
Bacterial promoter for expression of Kanr gene
-35 region: 3406-3411
-10 region: 3429-3434
Transcription start point: 3441
SV40 origin of replication: 3685-3820
SV40 early promoter
Enhancer (72-bp tandem repeats): 3518-3589 & 3590-3661
21-bp repeats: 3665-3685, 3686-3706 & 3708-3728
Early promoter element: 3741-3747
Major transcription start points: 3737, 3775, 3781 & 3786
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 3869-3871
Stop codon: 4661-4663
G->A mutation to remove Pst I site: 4051
C->A (Arg to Ser) mutation to remove BssH II site: 4397
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 4899-4904 & 4912-4917
pUC plasmid replication origin: 5248-5891


Notice to Purchaser:

mKate2-related materials (also referred to as "Products") are intended for research use only. The Products are covered by U.S. Pat. 7,638,615; European Pat. 1994149; and other Evrogen Patents and/or Patent applications pending. By use of these Products, you accept the terms and conditions of the applicable Limited Use Label License.

The CMV promoter is covered under U.S. Patents 5,168,062 and 5,385,839, and its use is permitted for research purposes only. Any other use of the CMV promoter requires a license from the University of Iowa Research Foundation, 214 Technology Innovation Center, Iowa City, IA 52242.

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