pmKate2-laminB1 vector

cat.# FP310

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

Download vector information: Data sheet (pdf) Sequence (txt) Restriction map (pdf) Restriction table (pdf)

Product Cat.# Size Price pmKate2-laminB1 FP310 20 μg € 400 The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information. Vector type mammalian expression vector Reporter mKate2 Reporter codon usage mammalian Promoter for mKate2 PCMV IE Host cells mammalian Selection prokaryotic – kanamycin eukaryotic – neomycin (G418) Replication prokaryotic – pUC ori eukaryotic – SV40 ori Use far-red fluorescent labeling of lamin B1

Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
Kozak consensus translation initiation site: 606-616
mKate2
Start codon (ATG): 613-615
Last amino acid in mKate2: 1312-1314
Lamin B1: 1345-3103
Stop codon: 3103-3105
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 3266-3271 & 3295-3300
mRNA 3' ends: 3304 & 3316
f1 single-strand DNA origin: 3363-3818
Bacterial promoter for expression of Kan^r gene
-35 region: 3880-3885
-10 region: 3903-3908
Transcription start point: 3915
SV40 origin of replication: 4159-4294
SV40 early promoter
Enhancer (72-bp tandem repeats): 3992-4063 & 4064-4135
21-bp repeats: 4139-4159, 4160-4180 & 4182-4202
Early promoter element: 4215-4221
Major transcription start points: 4211, 4249, 4255 & 4260
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 4343-4345
Stop codon: 5135-5137
G->A mutation to remove Pst I site: 4525
C->A (Arg to Ser) mutation to remove BssH II site: 4871
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 5373-5378 & 5386-5391
pUC plasmid replication origin: 5722-6365