pmKate2-paxillin vector

cat.# FP323

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

Download vector information: Data sheet (pdf) Sequence (txt) Restriction map (pdf) Restriction table (pdf)

Product Cat.# Size Price pmKate2-paxillin FP323 20 μg € 400 The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information. Vector type mammalian expression vector Reporter mKate2 Reporter codon usage mammalian Promoter for mKate2 PCMV IE Host cells mammalian Selection prokaryotic – kanamycin eukaryotic – neomycin (G418) Replication prokaryotic – pUC ori eukaryotic – SV40 ori Use far-red fluorescent labeling of paxillin

Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
Paxillin-mKate2 fusion: 600-3038
Paxillin: 600-2276
Start codon (ATG): 600-602
Last amino acid in Paxillin: 2274-2276
mKate2: 2343-3038
Stop codon: 3039-3041
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 3194-3199 & 3223-3228
mRNA 3' ends: 3232 & 3244
f1 single-strand DNA origin: 3291-3746
Bacterial promoter for expression of Kan^r gene
-35 region: 3808-3813
-10 region: 3831-3836
Transcription start point: 3843
SV40 origin of replication: 4087-4222
SV40 early promoter
Enhancer (72-bp tandem repeats): 3920-3991 & 3992-4063
21-bp repeats: 4067-4087, 4088-4108 & 4110-4130
Early promoter element: 4143-4149
Major transcription start points: 4139, 4177, 4183 & 4188
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 4271-4273
Stop codon: 5063-5065
G->A mutation to remove Pst I site: 4453
C->A (Arg to Ser) mutation to remove BssH II site: 4799
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 5301-5306 & 5314-5319
pUC plasmid replication origin: 5650-6293