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    pmKate2-profilin

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pmKate2-profilin vector

cat.# FP320

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

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vector information:
ProductCat.#SizePrice
pmKate2-profilinFP32020 μg€ 400
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Vector typemammalian expression vector
ReportermKate2
Reporter codon usagemammalian
Promoter for mKate2PCMV IE
Host cellsmammalian
Selectionprokaryotic – kanamycin
eukaryotic – neomycin (G418)
Replicationprokaryotic – pUC ori
eukaryotic – SV40 ori
Use far-red fluorescent labeling of profilin

Location of features

PCMV IE: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
mKate2-profilin fusion: 613-1764
mKate2: 613-1314
Start codon (ATG): 613-615
Last amino acid in mKate2: 1312-1314
Profilin: 1345-1764
Stop codon: 1765-1767
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 1928-1933 & 1957-1962
mRNA 3' ends: 1966 & 1978
f1 single-strand DNA origin: 2025-2480
Bacterial promoter for expression of Kanr gene
-35 region: 2542-2547
-10 region: 2565-2570
Transcription start point: 2577
SV40 origin of replication: 2821-2956
SV40 early promoter
Enhancer (72-bp tandem repeats): 2654-2725 & 2726-2797
21-bp repeats: 2801-2821, 2822-2842 & 2844-2864
Early promoter element: 2877-2883
Major transcription start points: 2873, 2911, 2917 & 2922
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 3005-3007
Stop codon: 3797-3799
G->A mutation to remove Pst I site: 3187
C->A (Arg to Ser) mutation to remove BssH II site: 3533
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 4035-4040 & 4048-4053
pUC plasmid replication origin: 4384-5027


Notice to Purchaser:

mKate2-related materials (also referred to as "Products") are intended for research use only. The Products are covered by U.S. Pat. 7,638,615; European Pat. 1994149; and other Evrogen Patents and/or Patent applications pending. By use of these Products, you accept the terms and conditions of the applicable Limited Use Label License.

The CMV promoter is covered under U.S. Patents 5,168,062 and 5,385,839, and its use is permitted for research purposes only. Any other use of the CMV promoter requires a license from the University of Iowa Research Foundation, 214 Technology Innovation Center, Iowa City, IA 52242.

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