pmKate2-zyxin vector

cat.# FP315

The vector sequence has been compiled using the information from sequence databases, published literature, and other sources, together with partial sequences obtained by Evrogen. This vector has not been completely sequenced.

Download vector information: Data sheet (pdf) Sequence (txt) Restriction map (pdf) Restriction table (pdf)

Product Cat.# Size Price pmKate2-zyxin FP315 20 μg € 400 The price does not include delivery. The price varies in different countries. Please contact your local distributor for exact prices and delivery information. Vector type mammalian expression vector Reporter mKate2 Reporter codon usage mammalian Promoter for mKate2 PCMV IE Host cells mammalian Selection prokaryotic – kanamycin eukaryotic – neomycin (G418) Replication prokaryotic – pUC ori eukaryotic – SV40 ori Use far-red fluorescent labeling of zyxin

Location of features

P_{CMV IE}: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
Zyxin: 636-2349
mKate2: 2370-3076
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 3221-3226 & 3250-3255
mRNA 3' ends: 3259 & 3271
f1 single-strand DNA origin: 3318-3773
Bacterial promoter for expression of Kan^r gene
-35 region: 3835-3840
-10 region: 3858-3863
Transcription start point: 3870
SV40 origin of replication: 4114-4249
SV40 early promoter
Enhancer (72-bp tandem repeats): 3947-4018 & 4019-4090
21-bp repeats: 4094-4114, 4115-4135 & 4137-4157
Early promoter element: 4170-4176
Major transcription start points: 4166, 4204, 4210 & 4215
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 4298-4300
Stop codon: 5090-5092
G->A mutation to remove Pst I site: 4480
C->A (Arg to Ser) mutation to remove BssH II site: 4826
Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal
Polyadenylation signals: 5328-5333 & 5341-5346
pUC plasmid replication origin: 5677-6320